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TitleHURP binding to the vinca domain of β-tubulin accounts for cancer drug resistance.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 8844, Year 2024
Publish dateOct 14, 2024
AuthorsAthira Saju / Po-Pang Chen / Tzu-Han Weng / Su-Yi Tsai / Akihiro Tanaka / Yu-Ting Tseng / Chih-Chia Chang / Chun-Hsiung Wang / Yuta Shimamoto / Kuo-Chiang Hsia /
PubMed AbstractVinca alkaloids, a class of tubulin-binding agent, are widely used in treating cancer, yet the emerging resistance compromises their efficacy. Hepatoma up-regulated protein (HURP), a microtubule- ...Vinca alkaloids, a class of tubulin-binding agent, are widely used in treating cancer, yet the emerging resistance compromises their efficacy. Hepatoma up-regulated protein (HURP), a microtubule-associated protein displaying heightened expression across various cancer types, reduces cancer cells' sensitivity to vinca-alkaloid drugs upon overexpression. However, the molecular basis behind this drug resistance remains unknown. Here we discover a tubulin-binding domain within HURP, and establish its role in regulating microtubule growth. Cryo-EM analysis reveals interactions between HURP's tubulin-binding domain and the vinca domain on β-tubulin -- the site targeted by vinca alkaloid drugs. Importantly, HURP competes directly with vinorelbine, a vinca alkaloid-based chemotherapeutic agent, countering microtubule growth defects caused by vinorelbine both in vitro and in vivo. Our findings elucidate a mechanism driving drug resistance in HURP-overexpressing cancer cells and emphasize HURP tubulin-binding domain's role in mitotic spindle assembly. This underscores its potential as a therapeutic target to improve cancer treatment.
External linksNat Commun / PubMed:39397030 / PubMed Central
MethodsEM (single particle)
Resolution3.54 - 3.59 Å
Structure data

38178

8x9p

EMDB-38178, PDB-8x9p:
HURP (428-534)-alpha-tubulin-beta-tubulin complex
Method: EM (single particle) / Resolution: 3.54 Å

EMDB-38179: Focus refinement of HURP-alpha-beta-tubulin
Method: EM (single particle) / Resolution: 3.54 Å

EMDB-38180: Focus refinement HURP-alpha-beta tubulin
Method: EM (single particle) / Resolution: 3.59 Å

EMDB-38181: HURP (428-534)-alpha-tubulin-beta-tubulin complex
Method: EM (single particle) / Resolution: 3.54 Å

Source
  • homo sapiens (human)
  • bos taurus (domestic cattle)
KeywordsCELL CYCLE / HURP / tubulin / drug resistance / mitosis

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