EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into transmembrane helix S0 facilitated RyR1 channel gating by Ca/ATP.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 1936, Year 2025
掲載日	2025年2月24日
著者	Risheng Wei / Qiang Chen / Lei Zhang / Congcong Liu / Chuang Liu / Chang-Cheng Yin / Hongli Hu /
PubMed 要旨	The type-1 ryanodine receptor (RyR1) is an intracellular calcium release channel for skeletal muscle excitation-contraction coupling. Previous structural studies showed that the RyR1 activity is ...The type-1 ryanodine receptor (RyR1) is an intracellular calcium release channel for skeletal muscle excitation-contraction coupling. Previous structural studies showed that the RyR1 activity is modulated by the exogenous regulators including caffeine, ryanodine, PCB-95 and diamide. An additional transmembrane helix, located adjacent to S1 and S4, has been observed in some structures, although its function remains unclear. Here, we report that using a mild purification procedure, this helix is co-purified with RyR1 and is designated as S0. When RyR1 is coupled with S0, it can be activated by Ca to an open state; however when decoupled from S0, it remains in primed state. S0 regulates the channel conformation by directly affecting the TM domain via the pVSD-S0-S4/S5 linker coupling, which facilitates the dilation of S6. Our results demonstrate that S0 is an essential component of RyR1 and plays a key role in the physiological regulation of RyR1 channel gating.
リンク	Nat Commun / PubMed:39994184 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 4.2 Å
構造データ	38042 8x48 EMDB-38042, PDB-8x48: Cryo-EM structure of Ryanodine receptor 1 (EGTA) 手法: EM (単粒子) / 解像度: 4.0 Å 38043 8x49 EMDB-38043, PDB-8x49: Cryo-EM structure of Ryanodine receptor 1 (100 nM Ca2+) 手法: EM (単粒子) / 解像度: 3.4 Å 38044 8x4a EMDB-38044, PDB-8x4a: Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, closed state) 手法: EM (単粒子) / 解像度: 3.8 Å 38045 8x4b EMDB-38045, PDB-8x4b: Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, open state) 手法: EM (単粒子) / 解像度: 4.2 Å 38046 8x4c EMDB-38046, PDB-8x4c: Cryo-EM structure of Ryanodine receptor 1 (20 uM Ca2+, 2 mM ATP) 手法: EM (単粒子) / 解像度: 3.8 Å 38047 8x4d EMDB-38047, PDB-8x4d: Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,20 uM Ca2+, 2 mM ATP) 手法: EM (単粒子) / 解像度: 3.9 Å 38048 8x4e EMDB-38048, PDB-8x4e: Cryo-EM structure of Ryanodine receptor 1 (TM helix S0, 5 mM Ca2+) 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM
由来	oryctolagus cuniculus (ウサギ)
キーワード	MEMBRANE PROTEIN / Ryanodine Receptor / Calcium release channel