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TitleStructural and mechanistic insights into symmetry conversion in plant GORK K+ channel regulation.
Journal, issue, pagesProtein Cell, Vol. 16, Issue 12, Page 1035-1047, Year 2025
Publish dateDec 1, 2025
AuthorsQi-Yu Li / Li Qin / Ling-Hui Tang / Chun-Rui Zhang / Shouguang Huang / Ke Wang / Gao-Hua Zhang / Ning-Jie Hao / Qian Xiao / Tongxin Niu / Min Su / Rainer Hedrich / Yu-Hang Chen /
PubMed AbstractGORK is a shaker-like potassium channel in plants that contains ankyrin (ANK) repeats. In guard cells, activation of GORK causes K+ efflux, reducing turgor pressure and closing stomata. However, how ...GORK is a shaker-like potassium channel in plants that contains ankyrin (ANK) repeats. In guard cells, activation of GORK causes K+ efflux, reducing turgor pressure and closing stomata. However, how GORK is regulated remains largely elusive. Here, we solved the cryo-EM structure of Arabidopsis GORK, revealing an unusual symmetry reduction (from C4 to C2) feature within its tetrameric assembly. This symmetry reduction in GORK channel is driven by ANK dimerization, which disrupts the coupling between transmembrane helices and cytoplasmic domains, thus maintaining GORK in an autoinhibited state. Electrophysiological and structural analyses further confirmed that ANK dimerization inhibits GORK, and its removal restores C4 symmetry, converting GORK to an activatable state. This dynamic switching between C2 and C4 symmetry, mediated by ANK dimerization, presents a GORK target site that guard cells regulate to switch the plant K+ channel between inhibited and activatable states, thus controlling stomatal movement in response to environmental stimuli.
External linksProtein Cell / PubMed:40996076 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 4.3 Å
Structure data

37500

8wfz

EMDB-37500, PDB-8wfz:
AtGORK Full length 2
Method: EM (single particle) / Resolution: 4.3 Å

62337

9khe

EMDB-62337, PDB-9khe:
AtGORK 1-623 truncated
Method: EM (single particle) / Resolution: 3.2 Å

62338

9khf

EMDB-62338, PDB-9khf:
AtGORK Full length 1
Method: EM (single particle) / Resolution: 3.4 Å

62339

9khg

EMDB-62339, PDB-9khg:
AtGORK 1-510 truncated
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-K:
Unknown entry

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / Complex / Protein / PLANT PROTEIN / PROTON TRANSPORT

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