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TitleAn intermediate open structure reveals the gating transition of the mechanically activated PIEZO1 channel.
Journal, issue, pagesNeuron, Year 2024
Publish dateDec 19, 2024
AuthorsSijia Liu / Xuzhong Yang / Xudong Chen / Xiaochun Zhang / Jinghui Jiang / Jingyi Yuan / Wenhao Liu / Li Wang / Heng Zhou / Kun Wu / Boxue Tian / Xueming Li / Bailong Xiao /
PubMed AbstractPIEZO1 is a mechanically activated cation channel that undergoes force-induced activation and inactivation. However, its distinct structural states remain undefined. Here, we employed an open-prone ...PIEZO1 is a mechanically activated cation channel that undergoes force-induced activation and inactivation. However, its distinct structural states remain undefined. Here, we employed an open-prone PIEZO1-S2472E mutant to capture an intermediate open structure. Compared with the curved and flattened structures of PIEZO1, the S2472E-Intermediate structure displays partially flattened blades, a downward and rotational motion of the top cap, and a spring-like compression of the linker connecting the cap to the pore-lining inner helix. These conformational changes open the cap gate and the hydrophobic transmembrane gate, whereas the intracellular lateral plug gate remains closed. The flattened structure of PIEZO1 with an up-state cap and closed cap gate might represent an inactivated state. Molecular dynamics (MD) simulations of ion conduction support the closed, intermediate open, and inactivated structural states. Mutagenesis and electrophysiological studies identified key domains and residues critical for the mechanical activation of PIEZO1. These studies collectively define the distinct structural states and gating transitions of PIEZO1.
External linksNeuron / PubMed:39719701
MethodsEM (single particle)
Resolution4.0 - 4.07 Å
Structure data

35799

8ixn

EMDB-35799, PDB-8ixn:
Curved structure of mPIEZO1-S2472E
Method: EM (single particle) / Resolution: 4.07 Å

35800

8ixo

EMDB-35800: Flattened structure of mPIEZO1-S2472E
PDB-8ixo: Intermediate structure of mPIEZO1-S2472E
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-36004: Intermediate structure of mPIEZO1-S2472E
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-PLX:
(9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL / phospholipid*YM

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM

ChemComp-P5S:
O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine

Source
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / Closed-state structure / PIEZO1 channel / Open-state structure

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