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Structure paper

TitleBoric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1.
Journal, issue, pagesNat Chem Biol, Vol. 20, Issue 5, Page 605-614, Year 2024
Publish dateJan 24, 2024
AuthorsMayuki Tanaka / Takeshi Yokoyama / Hironori Saito / Madoka Nishimoto / Kengo Tsuda / Naoyuki Sotta / Hideki Shigematsu / Mikako Shirouzu / Shintaro Iwasaki / Takuhiro Ito / Toru Fujiwara /
PubMed AbstractIn response to environmental changes, cells flexibly and rapidly alter gene expression through translational controls. In plants, the translation of NIP5;1, a boric acid diffusion facilitator, is ...In response to environmental changes, cells flexibly and rapidly alter gene expression through translational controls. In plants, the translation of NIP5;1, a boric acid diffusion facilitator, is downregulated in response to an excess amount of boric acid in the environment through upstream open reading frames (uORFs) that consist of only AUG and stop codons. However, the molecular details of how this minimum uORF controls translation of the downstream main ORF in a boric acid-dependent manner have remained unclear. Here, by combining ribosome profiling, translation complex profile sequencing, structural analysis with cryo-electron microscopy and biochemical assays, we show that the 80S ribosome assembled at AUG-stop migrates into the subsequent RNA segment, followed by downstream translation initiation, and that boric acid impedes this process by the stable confinement of eukaryotic release factor 1 on the 80S ribosome on AUG-stop. Our results provide molecular insight into translation regulation by a minimum and environment-responsive uORF.
External linksNat Chem Biol / PubMed:38267667
MethodsEM (single particle)
Resolution2.9 - 3.7 Å
Structure data

35634

8ip8

EMDB-35634, PDB-8ip8:
Wheat 80S ribosome stalled on AUG-Stop boron dependently
Method: EM (single particle) / Resolution: 2.9 Å

35635

8ip9

EMDB-35635, PDB-8ip9:
Wheat 40S ribosome in complex with a tRNAi
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-35636: Wheat 40S ribosome in complex with a tRNAi and eIF2
Method: EM (single particle) / Resolution: 3.7 Å

35637

8ipa

EMDB-35637, PDB-8ipa:
Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide
Method: EM (single particle) / Resolution: 3.4 Å

35638

8ipb

EMDB-35638, PDB-8ipb:
Wheat 80S ribosome pausing on AUG-Stop with cycloheximide
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione

Source
  • triticum aestivum (bread wheat)
  • oryza sativa (Asian cultivated rice)
KeywordsTRANSLATION / ribosome

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