EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational cycle of human polyamine transporter ATP13A2.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 1978, Year 2023
掲載日	2023年4月8日
著者	Jianqiang Mu / Chenyang Xue / Lei Fu / Zongjun Yu / Minhan Nie / Mengqi Wu / Xinmeng Chen / Kun Liu / Ruiqian Bu / Ying Huang / Baisheng Yang / Jianming Han / Qianru Jiang / Kevin C Chan / Ruhong Zhou / Huilin Li / Ancheng Huang / Yong Wang / Zhongmin Liu /
PubMed 要旨	Dysregulation of polyamine homeostasis strongly associates with human diseases. ATP13A2, which is mutated in juvenile-onset Parkinson's disease and autosomal recessive spastic paraplegia 78, is a ...Dysregulation of polyamine homeostasis strongly associates with human diseases. ATP13A2, which is mutated in juvenile-onset Parkinson's disease and autosomal recessive spastic paraplegia 78, is a transporter with a critical role in balancing the polyamine concentration between the lysosome and the cytosol. Here, to better understand human ATP13A2-mediated polyamine transport, we use single-particle cryo-electron microscopy to solve high-resolution structures of human ATP13A2 in six intermediate states, including the putative E2 structure for the P5 subfamily of the P-type ATPases. These structures comprise a nearly complete conformational cycle spanning the polyamine transport process and capture multiple substrate binding sites distributed along the transmembrane regions, suggesting a potential polyamine transport pathway. Integration of high-resolution structures, biochemical assays, and molecular dynamics simulations allows us to obtain a better understanding of the structural basis of how hATP13A2 transports polyamines, providing a mechanistic framework for ATP13A2-related diseases.
リンク	Nat Commun / PubMed:37031211 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 5.65 Å
構造データ	35384 8iek EMDB-35384, PDB-8iek: Cryo-EM structure of ATP13A2 in the E1-ATP state 手法: EM (単粒子) / 解像度: 3.2 Å 35385 8iel EMDB-35385, PDB-8iel: Cryo-EM structure of ATP13A2 in the E1-like state 手法: EM (単粒子) / 解像度: 5.65 Å 35386 8iem EMDB-35386, PDB-8iem: Cryo-EM structure of ATP13A2 in the E2P state 手法: EM (単粒子) / 解像度: 3.35 Å 35387 8ien EMDB-35387, PDB-8ien: Cryo-EM structure of ATP13A2 in the E2-Pi state 手法: EM (単粒子) / 解像度: 3.25 Å 35388 8ieo EMDB-35388, PDB-8ieo: Cryo-EM structure of ATP13A2 in the nominal E1P state 手法: EM (単粒子) / 解像度: 3.78 Å 35391 8ier EMDB-35391, PDB-8ier: Cryo-EM structure of ATP13A2 in the putative of E2 state 手法: EM (単粒子) / 解像度: 4.87 Å 35392 8ies EMDB-35392, PDB-8ies: Cryo-EM structure of ATP13A2 in the E1P-ADP state 手法: EM (単粒子) / 解像度: 3.73 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-SPM: SPERMINE / スペルミン / スペルミン ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	homo sapiens (ヒト)
キーワード	PROTEIN TRANSPORT / Cryo-EM structure of ATP13A2 in the E1-ATP state; Membran protein / Cryo-EM structure of ATP13A2 in the E1-like state / membrane protein (膜タンパク質) / Cryo-EM structure of ATP13A2 in the E2P state; Membran protein / TRANSPORT PROTEIN (運搬体タンパク質) / Cryo-EM structure of ATP13A2 in the E2-Pi state / Cryo-EM structure of ATP13A2 in the nominal E1P state; Membrane protein / Cryo-EM structure of ATP13A2 in the putative of E2 state; Membrane protein / Cryo-EM structure of ATP13A2 in the E1P-ADP state; Membrane protein