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-Structure paper
Title | Structural and functional properties of different types of siphonous LHCII trimers from an intertidal green alga Bryopsis corticulans. |
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Journal, issue, pages | Structure, Vol. 31, Issue 10, Page 1247-11258.e3, Year 2023 |
Publish date | Oct 5, 2023 |
![]() | Zhenhua Li / Cuicui Zhou / Songhao Zhao / Jinyang Zhang / Xueyang Liu / Min Sang / Xiaochun Qin / Yanyan Yang / Guangye Han / Tingyun Kuang / Jian-Ren Shen / Wenda Wang / ![]() ![]() |
PubMed Abstract | Light-harvesting complexes of photosystem II (LHCIIs) in green algae and plants are vital antenna apparatus for light harvesting, energy transfer, and photoprotection. Here we determined the ...Light-harvesting complexes of photosystem II (LHCIIs) in green algae and plants are vital antenna apparatus for light harvesting, energy transfer, and photoprotection. Here we determined the structure of a siphonous-type LHCII trimer from the intertidal green alga Bryopsis corticulans by X-ray crystallography and cryo-electron microscopy (cryo-EM), and analyzed its functional properties by spectral analysis. The Bryopsis LHCII (Bry-LHCII) structures in both homotrimeric and heterotrimeric form show that green light-absorbing siphonaxanthin and siphonein occupied the sites of lutein and violaxanthin in plant LHCII, and two extra chlorophylls (Chls) b replaced Chls a. Binding of these pigments expands the blue-green light absorption of B. corticulans in the tidal zone. We observed differences between the Bry-LHCII homotrimer crystal and cryo-EM structures, and also between Bry-LHCII homotrimer and heterotrimer cryo-EM structures. These conformational changes may reflect the flexibility of Bry-LHCII, which may be required to adapt to light fluctuations from tidal rhythms. |
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Methods | EM (single particle) / X-ray diffraction |
Resolution | 2.55 - 2.74 Å |
Structure data | EMDB-34883, PDB-8hlv: EMDB-34930, PDB-8hpd: ![]() PDB-8hq8: |
Chemicals | ![]() ChemComp-0UR: ![]() ChemComp-0IE: ![]() ChemComp-NEX: ![]() ChemComp-CHL: ![]() ChemComp-CLA: ![]() ChemComp-LHG: ![]() ChemComp-LMU: ![]() ChemComp-MG: ![]() ChemComp-BET: ![]() ChemComp-LMG: ![]() ChemComp-HOH: |
Source |
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![]() | PHOTOSYNTHESIS / light-harvesting complexes / siphonaxanthin / siphonein |