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TitleSix states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover.
Journal, issue, pagesCommun Biol, Vol. 6, Issue 1, Page 755, Year 2023
Publish dateJul 28, 2023
AuthorsRaymond N Burton-Smith / Chihong Song / Hiroshi Ueno / Takeshi Murata / Ryota Iino / Kazuyoshi Murata /
PubMed AbstractThe vacuolar-type ATPase from Enterococcus hirae (EhV-ATPase) is a thus-far unique adaptation of V-ATPases, as it performs Na transport and demonstrates an off-axis rotor assembly. Recent single ...The vacuolar-type ATPase from Enterococcus hirae (EhV-ATPase) is a thus-far unique adaptation of V-ATPases, as it performs Na transport and demonstrates an off-axis rotor assembly. Recent single molecule studies of the isolated V domain have indicated that there are subpauses within the three major states of the pseudo three-fold symmetric rotary enzyme. However, there was no structural evidence for these. Herein we activate the EhV-ATPase complex with ATP and identified multiple structures consisting of a total of six states of this complex by using cryo-electron microscopy. The orientations of the rotor complex during turnover, especially in the intermediates, are not as perfectly uniform as expected. The densities in the nucleotide binding pockets in the V domain indicate the different catalytic conditions for the six conformations. The off-axis rotor and its' interactions with the stator a-subunit during rotation suggests that this non-uniform rotor rotation is performed through the entire complex.
External linksCommun Biol / PubMed:37507515 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 7.7 Å
Structure data

EMDB-34139: E. hirae V-ATPase state 1 (whole complex)
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-34140: E. hirae V-ATPase state 1' (whole complex)
Method: EM (single particle) / Resolution: 7.7 Å

EMDB-34141: E. hirae V-ATPase state 2 (whole complex)
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-34142: E. hirae V-ATPase state 2' (whole complex)
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-34143: E. hirae V-ATPase state 3 (whole complex)
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-34144: E. hirae V-ATPase state 3' (whole complex)
Method: EM (single particle) / Resolution: 7.3 Å

EMDB-34145: E. hirae V-ATPase state 1 (V1 domain)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-34146: E. hirae V-ATPase state 1' (V1 domain)
Method: EM (single particle) / Resolution: 7.7 Å

EMDB-34147: E. hirae V-ATPase state 2 (V1 domain)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-34148: E. hirae V-ATPase state 2' (V1 domain)
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-34149: E. hirae V-ATPase state 3 (V1 domain)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-34150: E. hirae V-ATPase state 3' (V1 domain)
Method: EM (single particle) / Resolution: 7.0 Å

Source
  • Enterococcus hirae ATCC 9790 (bacteria)

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