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TitleDesigner nanoscale DNA assemblies programmed from the top down.
Journal, issue, pagesScience, Vol. 352, Issue 6293, Page 1534, Year 2016
Publish dateJun 24, 2016
AuthorsRémi Veneziano / Sakul Ratanalert / Kaiming Zhang / Fei Zhang / Hao Yan / Wah Chiu / Mark Bathe /
PubMed AbstractScaffolded DNA origami is a versatile means of synthesizing complex molecular architectures. However, the approach is limited by the need to forward-design specific Watson-Crick base pairing manually ...Scaffolded DNA origami is a versatile means of synthesizing complex molecular architectures. However, the approach is limited by the need to forward-design specific Watson-Crick base pairing manually for any given target structure. Here, we report a general, top-down strategy to design nearly arbitrary DNA architectures autonomously based only on target shape. Objects are represented as closed surfaces rendered as polyhedral networks of parallel DNA duplexes, which enables complete DNA scaffold routing with a spanning tree algorithm. The asymmetric polymerase chain reaction is applied to produce stable, monodisperse assemblies with custom scaffold length and sequence that are verified structurally in three dimensions to be high fidelity by single-particle cryo-electron microscopy. Their long-term stability in serum and low-salt buffer confirms their utility for biological as well as nonbiological applications.
External linksScience / PubMed:27229143 / PubMed Central
MethodsEM (single particle)
Resolution20.0 - 40.0 Å
Structure data

EMDB-3408:
cryo-EM of nanoscale DNA assemblies
Method: EM (single particle) / Resolution: 22.0 Å

EMDB-3409:
cryo-EM of nanoscale DNA assemblies
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-3410:
cryo-EM of nanoscale DNA assemblies
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-3411:
cryo-EM of nanoscale DNA assemblies
Method: EM (single particle) / Resolution: 29.0 Å

EMDB-3412:
cryo-EM of nanoscale DNA assemblies
Method: EM (single particle) / Resolution: 27.0 Å

EMDB-3413:
cryo-EM of nanoscale DNA assemblies
Method: EM (single particle) / Resolution: 40.0 Å

Source
  • Enterobacteria phage M13 (virus)
  • synthetic construct (others)

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