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Title3D RNA-scaffolded wireframe origami.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 382, Year 2023
Publish dateJan 24, 2023
AuthorsMolly F Parsons / Matthew F Allan / Shanshan Li / Tyson R Shepherd / Sakul Ratanalert / Kaiming Zhang / Krista M Pullen / Wah Chiu / Silvi Rouskin / Mark Bathe /
PubMed AbstractHybrid RNA:DNA origami, in which a long RNA scaffold strand folds into a target nanostructure via thermal annealing with complementary DNA oligos, has only been explored to a limited extent despite ...Hybrid RNA:DNA origami, in which a long RNA scaffold strand folds into a target nanostructure via thermal annealing with complementary DNA oligos, has only been explored to a limited extent despite its unique potential for biomedical delivery of mRNA, tertiary structure characterization of long RNAs, and fabrication of artificial ribozymes. Here, we investigate design principles of three-dimensional wireframe RNA-scaffolded origami rendered as polyhedra composed of dual-duplex edges. We computationally design, fabricate, and characterize tetrahedra folded from an EGFP-encoding messenger RNA and de Bruijn sequences, an octahedron folded with M13 transcript RNA, and an octahedron and pentagonal bipyramids folded with 23S ribosomal RNA, demonstrating the ability to make diverse polyhedral shapes with distinct structural and functional RNA scaffolds. We characterize secondary and tertiary structures using dimethyl sulfate mutational profiling and cryo-electron microscopy, revealing insight into both global and local, base-level structures of origami. Our top-down sequence design strategy enables the use of long RNAs as functional scaffolds for complex wireframe origami.
External linksNat Commun / PubMed:36693871 / PubMed Central
MethodsEM (single particle)
Resolution12.0 - 19.0 Å
Structure data

EMDB-34058: rO44 A-form (staple xover asymmetry)
Method: EM (single particle) / Resolution: 17.0 Å

EMDB-34059: rO66 A-form (staple xover asymmetry)
Method: EM (single particle) / Resolution: 13.0 Å

EMDB-34060: rT66 A-form (staple xover asymmetry)
Method: EM (single particle) / Resolution: 12.0 Å

EMDB-34061: rPB66 A-form (staple xover asymmetry)
Method: EM (single particle) / Resolution: 19.0 Å

Source
  • Inovirus M13

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