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TitleAsymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ.
Journal, issue, pagesNat Commun, Vol. 7, Page 12524, Year 2016
Publish dateAug 26, 2016
AuthorsRoman I Koning / Josue Gomez-Blanco / Inara Akopjana / Javier Vargas / Andris Kazaks / Kaspars Tars / José María Carazo / Abraham J Koster /
PubMed AbstractIn single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the ...In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the asymmetric virion structure of bacteriophage MS2, which includes 178 copies of the coat protein, a single copy of the A-protein and the RNA genome. This reveals that in situ, the viral RNA genome can adopt a defined conformation. The RNA forms a branched network of stem-loops that almost all allocate near the capsid inner surface, while predominantly binding to coat protein dimers that are located in one-half of the capsid. This suggests that genomic RNA is highly involved in genome packaging and virion assembly.
External linksNat Commun / PubMed:27561669 / PubMed Central
MethodsEM (single particle)
Resolution5.0 - 10.5 Å
Structure data

EMDB-3402:
Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ
Method: EM (single particle) / Resolution: 5.0 Å

EMDB-3403:
Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ
Method: EM (single particle) / Resolution: 10.5 Å

EMDB-3404:
Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ
Method: EM (single particle) / Resolution: 10.5 Å

EMDB-3540:
Asymmetric reconstruction of MS2 virion
Method: EM (single particle) / Resolution: 7.9 Å

Source
  • Enterobacterio phage MS2 (virus)

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