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-Structure paper
Title | Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ. |
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Journal, issue, pages | Nat Commun, Vol. 7, Page 12524, Year 2016 |
Publish date | Aug 26, 2016 |
![]() | Roman I Koning / Josue Gomez-Blanco / Inara Akopjana / Javier Vargas / Andris Kazaks / Kaspars Tars / José María Carazo / Abraham J Koster / ![]() ![]() ![]() |
PubMed Abstract | In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the ...In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the asymmetric virion structure of bacteriophage MS2, which includes 178 copies of the coat protein, a single copy of the A-protein and the RNA genome. This reveals that in situ, the viral RNA genome can adopt a defined conformation. The RNA forms a branched network of stem-loops that almost all allocate near the capsid inner surface, while predominantly binding to coat protein dimers that are located in one-half of the capsid. This suggests that genomic RNA is highly involved in genome packaging and virion assembly. |
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Methods | EM (single particle) |
Resolution | 5.0 - 10.5 Å |
Structure data | ![]() EMDB-3402: ![]() EMDB-3403: ![]() EMDB-3404: ![]() EMDB-3540: |
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