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-Structure paper
Title | Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA. |
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Journal, issue, pages | Nat Commun, Vol. 13, Issue 1, Page 4668, Year 2022 |
Publish date | Aug 15, 2022 |
Authors | Seungha Hwang / Paul Dominic B Olinares / Jimin Lee / Jinwoo Kim / Brian T Chait / Rodney A King / Jin Young Kang / |
PubMed Abstract | Transcription, in which RNA polymerases (RNAPs) produce RNA from DNA, is the first step of gene expression. As such, it is highly regulated either by trans-elements like protein factors and/or by cis- ...Transcription, in which RNA polymerases (RNAPs) produce RNA from DNA, is the first step of gene expression. As such, it is highly regulated either by trans-elements like protein factors and/or by cis-elements like specific sequences on the DNA. Lambdoid phage HK022 contains a cis-element, put, which suppresses pausing and termination during transcription of the early phage genes. The putRNA transcript solely performs the anti-pausing/termination activities by interacting directly with the E.coli RNAP elongation complex (EC) by an unknown structural mechanism. In this study, we reconstituted putRNA-associated ECs and determined the structures using cryo-electron microscopy. The determined structures of putRNA-associated EC, putRNA-absent EC, and σ-bound EC suggest that the putRNA interaction with the EC counteracts swiveling, a conformational change previously identified to promote pausing and σ might modulate putRNA folding via σ-dependent pausing during elongation. |
External links | Nat Commun / PubMed:35970830 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.17 - 3.61 Å |
Structure data | EMDB-33466, PDB-7xue: EMDB-33468, PDB-7xug: EMDB-33470, PDB-7xui: |
Chemicals | ChemComp-ZN: ChemComp-MG: |
Source |
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Keywords | TRANSCRIPTION / RNA polymerase / anti-pausing / anti-termination / cryo-EM / HK022 put / bacterial transcription |