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TitleA Neutralizing Antibody Targeting gH Provides Potent Protection against EBV Challenge .
Journal, issue, pagesJ Virol, Vol. 96, Issue 8, Page e0007522, Year 2022
Publish dateApr 27, 2022
AuthorsJunping Hong / Ling Zhong / Qingbing Zheng / Qian Wu / Zhenghui Zha / Dongmei Wei / Haiwen Chen / Wanlin Zhang / Shanshan Zhang / Yang Huang / Kaiyun Chen / Junyu Chen / Shaowei Li / Mu-Sheng Zeng / Yi-Xin Zeng / Ningshao Xia / Xiao Zhang / Miao Xu / Yixin Chen /
PubMed AbstractEpstein-Barr virus (EBV) is an oncogenic herpesvirus that is associated with 200,000 new cases of cancer and 140,000 deaths annually. To date, there are no available vaccines or therapeutics for ...Epstein-Barr virus (EBV) is an oncogenic herpesvirus that is associated with 200,000 new cases of cancer and 140,000 deaths annually. To date, there are no available vaccines or therapeutics for clinical usage. Recently, the viral heterodimer glycoprotein gH/gL has become a promising target for the development of prophylactic vaccines against EBV. Here, we developed the anti-gH antibody 6H2 and its chimeric version C6H2, which had full neutralizing activity in epithelial cells and partial neutralizing activity in B cells. C6H2 exhibited potent protection against lethal EBV challenge in a humanized mouse model. The cryo-electron microscopy (cryo-EM) structure further revealed that 6H2 recognized a previously unidentified epitope on gH/gL D-IV that is critical for viral attachment and subsequent membrane fusion with epithelial cells. Our results suggest that C6H2 is a promising candidate in the prevention of EBV-induced lymphoproliferative diseases (LPDs) and may inform the design of an EBV vaccine. Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that establishes lifelong persistence and is related to multiple diseases, including cancers. Neutralizing antibodies (NAbs) have proven to be highly effective in preventing EBV infection and subsequent diseases. Here, we developed an anti-EBV-gH NAb, 6H2, which blocked EBV infection and . This 6H2 neutralizing epitope should be helpful to understand EBV infection mechanisms and guide the development of vaccines and therapeutics against EBV infection.
External linksJ Virol / PubMed:35348362 / PubMed Central
MethodsEM (single particle)
Resolution6.87 Å
Structure data

EMDB-33102: Cryo-EM structure of EBV glycoprotein complex gHgL-gp42 bound by a neutralizing antibody 6H2
Method: EM (single particle) / Resolution: 6.87 Å

Source
  • Human gammaherpesvirus 4 (Epstein-Barr virus)
  • Oryctolagus cuniculus (rabbit)

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