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TitleA prototype protein nanocage minimized from carboxysomes with gated oxygen permeability.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 119, Issue 5, Year 2022
Publish dateFeb 1, 2022
AuthorsRuimin Gao / Huan Tan / Shanshan Li / Shaojie Ma / Yufu Tang / Kaiming Zhang / Zhiping Zhang / Quli Fan / Jun Yang / Xian-En Zhang / Feng Li /
PubMed AbstractProtein nanocages (PNCs) in cells and viruses have inspired the development of self-assembling protein nanomaterials for various purposes. Despite the successful creation of artificial PNCs, the de ...Protein nanocages (PNCs) in cells and viruses have inspired the development of self-assembling protein nanomaterials for various purposes. Despite the successful creation of artificial PNCs, the de novo design of PNCs with defined permeability remains challenging. Here, we report a prototype oxygen-impermeable PNC (OIPNC) assembled from the vertex protein of the β-carboxysome shell, CcmL, with quantum dots as the template via interfacial engineering. The structure of the cage was solved at the atomic scale by combined solid-state NMR spectroscopy and cryoelectron microscopy, showing icosahedral assembly of CcmL pentamers with highly conserved interpentamer interfaces. Moreover, a gating mechanism was established by reversibly blocking the pores of the cage with molecular patches. Thus, the oxygen permeability, which was probed by an oxygen sensor inside the cage, can be completely controlled. The CcmL OIPNC represents a PNC platform for oxygen-sensitive or oxygen-responsive storage, catalysis, delivery, sensing, etc.
External linksProc Natl Acad Sci U S A / PubMed:35078933 / PubMed Central
MethodsEM (single particle) / NMR (solid-state)
Resolution9.2 Å
Structure data

EMDB-32549:
Cryo-EM Structure QD@HCcmL complex
Method: EM (single particle) / Resolution: 9.2 Å

PDB-7wkc:
A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability
Method: SOLID-STATE NMR

Source
  • Thermosynechococcus elongatus BP-1 (bacteria)
  • thermosynechococcus vestitus bp-1 (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / protein cages / quantum dots / Cryoelectron microscopy

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