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TitleThe lysosomal transporter TAPL has a dual role as peptide translocator and phosphatidylserine floppase.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 5851, Year 2022
Publish dateOct 4, 2022
AuthorsJun Gyou Park / Songwon Kim / Eunhong Jang / Seung Hun Choi / Hyunsu Han / Seulgi Ju / Ji Won Kim / Da Sol Min / Mi Sun Jin /
PubMed AbstractTAPL is a lysosomal ATP-binding cassette transporter that translocates a broad spectrum of polypeptides from the cytoplasm into the lysosomal lumen. Here we report that, in addition to its well-known ...TAPL is a lysosomal ATP-binding cassette transporter that translocates a broad spectrum of polypeptides from the cytoplasm into the lysosomal lumen. Here we report that, in addition to its well-known role as a peptide translocator, TAPL exhibits an ATP-dependent phosphatidylserine floppase activity that is the possible cause of its high basal ATPase activity and of the lack of coupling between ATP hydrolysis and peptide efflux. We also present the cryo-EM structures of mouse TAPL complexed with (i) phospholipid, (ii) cholesteryl hemisuccinate (CHS) and 9-mer peptide, and (iii) ADP·BeF. The inward-facing structure reveals that F449 protrudes into the cylindrical transport pathway and divides it into a large hydrophilic central cavity and a sizable hydrophobic upper cavity. In the structure, the peptide binds to TAPL in horizontally-stretched fashion within the central cavity, while lipid molecules plug vertically into the upper cavity. Together, our results suggest that TAPL uses different mechanisms to function as a peptide translocase and a phosphatidylserine floppase.
External linksNat Commun / PubMed:36195619 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.98 Å
Structure data

31722

7v5c

EMDB-31722, PDB-7v5c:
Cryo-EM structure of the mouse ABCB9 (ADP.BeF3-bound)
Method: EM (single particle) / Resolution: 3.2 Å

31723

7v5d

EMDB-31723, PDB-7v5d:
Cryo-EM structure of the mouse ABCB9 (PG-bound)
Method: EM (single particle) / Resolution: 3.4 Å

31955

7vfi

EMDB-31955, PDB-7vfi:
Cryo-EM structure of the mouse TAPL (9mer-peptide bound)
Method: EM (single particle) / Resolution: 3.98 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-MG:
Unknown entry

ChemComp-PGT:
(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / phospholipid*YM

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

Source
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / ABCB9 / peptide transporter / Lipid floppase / TAPL

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