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TitleStructural basis of transcription activation by the global regulator Spx.
Journal, issue, pagesNucleic Acids Res, Vol. 49, Issue 18, Page 10756-10769, Year 2021
Publish dateOct 11, 2021
AuthorsJing Shi / Fangfang Li / Aijia Wen / Libing Yu / Lu Wang / Fulin Wang / Yuanling Jin / Sha Jin / Yu Feng / Wei Lin /
PubMed AbstractSpx is a global transcriptional regulator in Gram-positive bacteria and has been inferred to efficiently activate transcription upon oxidative stress by engaging RNA polymerase (RNAP) and promoter ...Spx is a global transcriptional regulator in Gram-positive bacteria and has been inferred to efficiently activate transcription upon oxidative stress by engaging RNA polymerase (RNAP) and promoter DNA. However, the precise mechanism by which it interacts with RNAP and promoter DNA to initiate transcription remains obscure. Here, we report the cryo-EM structure of an intact Spx-dependent transcription activation complex (Spx-TAC) from Bacillus subtilis at 4.2 Å resolution. The structure traps Spx in an active conformation and defines key interactions accounting for Spx-dependent transcription activation. Strikingly, an oxidized Spx monomer engages RNAP by simultaneously interacting with the C-terminal domain of RNAP alpha subunit (αCTD) and σA. The interface between Spx and αCTD is distinct from those previously reported activators, indicating αCTD as a multiple target for the interaction between RNAP and various transcription activators. Notably, Spx specifically wraps the conserved -44 element of promoter DNA, thereby stabilizing Spx-TAC. Besides, Spx interacts extensively with σA through three different interfaces and promotes Spx-dependent transcription activation. Together, our structural and biochemical results provide a novel mechanistic framework for the regulation of bacterial transcription activation and shed new light on the physiological roles of the global Spx-family transcription factors.
External linksNucleic Acids Res / PubMed:34530448 / PubMed Central
MethodsEM (single particle)
Resolution4.2 Å
Structure data

31485

7f75

EMDB-31485, PDB-7f75:
Cryo-EM structure of Spx-dependent transcription activation complex
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • bacillus subtilis (bacteria)
KeywordsTRANSCRIPTION/DNA / Complex / transcription activator / stress / oxidated form / TRANSCRIPTION / TRANSCRIPTION-DNA complex

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