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Structure paper

TitleStructural basis for the recognition of SARS-CoV-2 by full-length human ACE2.
Journal, issue, pagesScience, Vol. 367, Issue 6485, Page 1444-1448, Year 2020
Publish dateMar 27, 2020
AuthorsRenhong Yan / Yuanyuan Zhang / Yaning Li / Lu Xia / Yingying Guo / Qiang Zhou /
PubMed AbstractAngiotensin-converting enzyme 2 (ACE2) is the cellular receptor for severe acute respiratory syndrome-coronavirus (SARS-CoV) and the new coronavirus (SARS-CoV-2) that is causing the serious ...Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for severe acute respiratory syndrome-coronavirus (SARS-CoV) and the new coronavirus (SARS-CoV-2) that is causing the serious coronavirus disease 2019 (COVID-19) epidemic. Here, we present cryo-electron microscopy structures of full-length human ACE2 in the presence of the neutral amino acid transporter BAT1 with or without the receptor binding domain (RBD) of the surface spike glycoprotein (S protein) of SARS-CoV-2, both at an overall resolution of 2.9 angstroms, with a local resolution of 3.5 angstroms at the ACE2-RBD interface. The ACE2-BAT1 complex is assembled as a dimer of heterodimers, with the collectrin-like domain of ACE2 mediating homodimerization. The RBD is recognized by the extracellular peptidase domain of ACE2 mainly through polar residues. These findings provide important insights into the molecular basis for coronavirus recognition and infection.
External linksScience / PubMed:32132184 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 4.5 Å
Structure data

30039

6m17

EMDB-30039, PDB-6m17:
The 2019-nCoV RBD/ACE2-B0AT1 complex
Method: EM (single particle) / Resolution: 2.9 Å

30040

6m18

EMDB-30040, PDB-6m18:
ACE2-B0AT1 complex
Method: EM (single particle) / Resolution: 2.9 Å

30041

6m1d

EMDB-30041, PDB-6m1d:
ACE2-B0AT1 complex, open conformation
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-30042:
cryo_EM map of the complex of the RBD of 2019-nCoV with the ACE2-B0AT1 complex, focused refined on extracellular region of ACE2
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-30043:
Cryo_EM map of the complex of the RBD of 2019-nCoV with the ACE2-B0AT1 complex, focused refined on transmembrane region of ACE2-B0AT1 complex
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-30044:
cryo EM map of ACE2-B0AT1 complex, focused refined on extracellular region
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-30045:
Cryo EM map of the ACE2-B0AT1 complex, focused refined on transmembrane region
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-30046:
Cryo EM map of the complex of the RBD of 2019-nCoV with the ACE2-B0AT1 complex, focused refined at the interface between RBD and ACE2
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-31252: cryo EM map of Erastin-bound xCT-4F2hc complex, focused refined on transmembrane region
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-LEU:
LEUCINE / Leucine

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / Phosphatidic acid

Source
  • severe acute respiratory syndrome coronavirus 2
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN/VIRAL PROTEIN / 2019-nCoV RBD / ACE2-B0AT1 complex / MEMBRANE PROTEIN / MEMBRANE PROTEIN-VIRAL PROTEIN complex

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