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TitleIn situ cryo-ET structure of phycobilisome-photosystem II supercomplex from red alga.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateSep 13, 2021
AuthorsMeijing Li / Jianfei Ma / Xueming Li / Sen-Fang Sui /
PubMed AbstractPhycobilisome (PBS) is the main light-harvesting antenna in cyanobacteria and red algae. How PBS transfers the light energy to photosystem II (PSII) remains to be elucidated. Here we report the in ...Phycobilisome (PBS) is the main light-harvesting antenna in cyanobacteria and red algae. How PBS transfers the light energy to photosystem II (PSII) remains to be elucidated. Here we report the in situ structure of the PBS-PSII supercomplex from UTEX 2757 using cryo-electron tomography and subtomogram averaging. Our work reveals the organized network of hemiellipsoidal PBS with PSII on the thylakoid membrane in the native cellular environment. In the PBS-PSII supercomplex, each PBS interacts with six PSII monomers, of which four directly bind to the PBS, and two bind indirectly. Additional three 'connector' proteins also contribute to the connections between PBS and PSIIs. Two PsbO subunits from adjacent PSII dimers bind with each other, which may promote stabilization of the PBS-PSII supercomplex. By analyzing the interaction interface between PBS and PSII, we reveal that α and ApcD connect with CP43 of PSII monomer and that α also interacts with CP47' of the neighboring PSII monomer, suggesting the multiple light energy delivery pathways. The in situ structures illustrate the coupling pattern of PBS and PSII and the arrangement of the PBS-PSII supercomplex on the thylakoid, providing the near-native 3D structural information of the various energy transfer from PBS to PSII.
External linksElife / PubMed:34515634 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution13.2 - 15.6 Å
Structure data

EMDB-31241:
Structure of the PBS-PSII supercomplex from red algae
Method: EM (subtomogram averaging) / Resolution: 13.2 Å

EMDB-31242:
Structure of the double phycobilisome-photosystem II supercomplex from red alga
Method: EM (subtomogram averaging) / Resolution: 15.6 Å

EMDB-31243:
Cryo-electron tomography of phycobilisome-photosystem II supercomplex on the thylakoid
Method: EM (tomography)

EMDB-31244:
Cryo-electron tomogram of phycobilisome-photosystem II supercomplex on the thylakoid
Method: EM (tomography)

EMDB-31245:
Cryo-electron tomogram of phycobilisome-photosystem II supercomplex on the thylakoid
Method: EM (tomography)

EMDB-31247:
Cryo-electron tomogram of phycobilisome-photosystem II supercomplex on the thylakoid
Method: EM (tomography)

Source
  • Porphyridium purpureum (eukaryote)

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