+検索条件
-Structure paper
| タイトル | In situ cryo-ET structure of phycobilisome-photosystem II supercomplex from red alga. |
|---|---|
| ジャーナル・号・ページ | Elife, Vol. 10, Year 2021 |
| 掲載日 | 2021年9月13日 |
著者 | Meijing Li / Jianfei Ma / Xueming Li / Sen-Fang Sui / ![]() |
| PubMed 要旨 | Phycobilisome (PBS) is the main light-harvesting antenna in cyanobacteria and red algae. How PBS transfers the light energy to photosystem II (PSII) remains to be elucidated. Here we report the in ...Phycobilisome (PBS) is the main light-harvesting antenna in cyanobacteria and red algae. How PBS transfers the light energy to photosystem II (PSII) remains to be elucidated. Here we report the in situ structure of the PBS-PSII supercomplex from UTEX 2757 using cryo-electron tomography and subtomogram averaging. Our work reveals the organized network of hemiellipsoidal PBS with PSII on the thylakoid membrane in the native cellular environment. In the PBS-PSII supercomplex, each PBS interacts with six PSII monomers, of which four directly bind to the PBS, and two bind indirectly. Additional three 'connector' proteins also contribute to the connections between PBS and PSIIs. Two PsbO subunits from adjacent PSII dimers bind with each other, which may promote stabilization of the PBS-PSII supercomplex. By analyzing the interaction interface between PBS and PSII, we reveal that α and ApcD connect with CP43 of PSII monomer and that α also interacts with CP47' of the neighboring PSII monomer, suggesting the multiple light energy delivery pathways. The in situ structures illustrate the coupling pattern of PBS and PSII and the arrangement of the PBS-PSII supercomplex on the thylakoid, providing the near-native 3D structural information of the various energy transfer from PBS to PSII. |
リンク | Elife / PubMed:34515634 / PubMed Central |
| 手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
| 解像度 | 13.2 - 15.6 Å |
| 構造データ | ![]() EMDB-31241: ![]() EMDB-31242: ![]() EMDB-31243: ![]() EMDB-31244: ![]() EMDB-31245: ![]() EMDB-31247: |
| 由来 |
|
ムービー
コントローラー
構造ビューア
万見文献について



著者
リンク





Porphyridium purpureum (真核生物)