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TitleThe cryo-EM structure of the chloroplast ClpP complex.
Journal, issue, pagesNat Plants, Vol. 7, Issue 11, Page 1505-1515, Year 2021
Publish dateNov 15, 2021
AuthorsNing Wang / Yifan Wang / Qian Zhao / Xiang Zhang / Chao Peng / Wenjuan Zhang / Yanan Liu / Olivier Vallon / Michael Schroda / Yao Cong / Cuimin Liu /
PubMed AbstractProtein homoeostasis in plastids is strategically regulated by the protein quality control system involving multiple chaperones and proteases, among them the Clp protease. Here, we determined the ...Protein homoeostasis in plastids is strategically regulated by the protein quality control system involving multiple chaperones and proteases, among them the Clp protease. Here, we determined the structure of the chloroplast ClpP complex from Chlamydomonas reinhardtii by cryo-electron microscopy. ClpP contains two heptameric catalytic rings without any symmetry. The top ring contains one ClpR6, three ClpP4 and three ClpP5 subunits while the bottom ring is composed of three ClpP1 subunits and one each of the ClpR1-4 subunits. ClpR3, ClpR4 and ClpT4 subunits connect the two rings and stabilize the complex. The chloroplast Cpn11/20/23 co-chaperonin, a co-factor of Cpn60, forms a cap on the top of ClpP by protruding mobile loops into hydrophobic clefts at the surface of the top ring. The co-chaperonin repressed ClpP proteolytic activity in vitro. By regulating Cpn60 chaperone and ClpP protease activity, the co-chaperonin may play a role in coordinating protein folding and degradation in the chloroplast.
External linksNat Plants / PubMed:34782772
MethodsEM (single particle)
Resolution3.3 - 4.8 Å
Structure data

31171

7eko

EMDB-31171, PDB-7eko:
CrClpP-S1
Method: EM (single particle) / Resolution: 3.3 Å

31173

7ekq

EMDB-31173, PDB-7ekq:
CrClpP-S2c
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-31174:
Cpn11/20/23
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-31175:
ClpP-S2
Method: EM (single particle) / Resolution: 3.6 Å

Source
  • chlamydomonas reinhardtii (plant)
  • Chlamydomonas smithii (plant)
KeywordsSTRUCTURAL PROTEIN / Clp / Complex / Protease / Chloroplast / Chlamydomnas / Atp-dependent.

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