Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of assembly and torque transmission of the bacterial flagellar motor.
Journal, issue, pages	Cell, Vol. 184, Issue 10, Page 2665-2679.e19, Year 2021
Publish date	May 13, 2021
Authors	Jiaxing Tan / Xing Zhang / Xiaofei Wang / Caihuang Xu / Shenghai Chang / Hangjun Wu / Ting Wang / Huihui Liang / Haichun Gao / Yan Zhou / Yongqun Zhu /
PubMed Abstract	The bacterial flagellar motor is a supramolecular protein machine that drives rotation of the flagellum for motility, which is essential for bacterial survival in different environments and a key ...The bacterial flagellar motor is a supramolecular protein machine that drives rotation of the flagellum for motility, which is essential for bacterial survival in different environments and a key determinant of pathogenicity. The detailed structure of the flagellar motor remains unknown. Here we present an atomic-resolution cryoelectron microscopy (cryo-EM) structure of the bacterial flagellar motor complexed with the hook, consisting of 175 subunits with a molecular mass of approximately 6.3 MDa. The structure reveals that 10 peptides protruding from the MS ring with the FlgB and FliE subunits mediate torque transmission from the MS ring to the rod and overcome the symmetry mismatch between the rotational and helical structures in the motor. The LP ring contacts the distal rod and applies electrostatic forces to support its rotation and torque transmission to the hook. This work provides detailed molecular insights into the structure, assembly, and torque transmission mechanisms of the flagellar motor.
External links	Cell / PubMed:33882274
Methods	EM (single particle)
Resolution	2.8 - 4.5 Å
Structure data	30335 7cbl EMDB-30335, PDB-7cbl: Cryo-EM structure of the flagellar LP ring from Salmonella Method: EM (single particle) / Resolution: 2.8 Å 30336 7cbm EMDB-30336, PDB-7cbm: Cryo-EM structure of the flagellar distal rod with partial hook from Salmonella Method: EM (single particle) / Resolution: 3.2 Å 30348 7cg0 EMDB-30348, PDB-7cg0: Cryo-EM structure of the flagellar proximal rod with FliF peptides from Salmonella Method: EM (single particle) / Resolution: 3.2 Å 30350 7cg4 EMDB-30350, PDB-7cg4: Cryo-EM structure of the flagellar export apparatus with FliE from Salmonella Method: EM (single particle) / Resolution: 3.6 Å 30351 7cg7 EMDB-30351, PDB-7cg7: Cryo-EM structure of the flagellar MS ring with C34 symmetry from Salmonella Method: EM (single particle) / Resolution: 3.61 Å 30354 7cgb EMDB-30354, PDB-7cgb: Cryo-EM structure of the flagellar hook from Salmonella Method: EM (single particle) / Resolution: 3.4 Å 30359 7cgo EMDB-30359, PDB-7cgo: Cryo-EM structure of the flagellar motor-hook complex from Salmonella Method: EM (single particle) / Resolution: 3.9 Å 31006 7e80 EMDB-31006, PDB-7e80: Cryo-EM structure of the flagellar rod with hook and export apparatus from Salmonella Method: EM (single particle) / Resolution: 3.67 Å 31007 7e81 EMDB-31007, PDB-7e81: Cryo-EM structure of the flagellar MS ring with FlgB-Dc loop and FliE-helix 1 from Salmonella Method: EM (single particle) / Resolution: 4.5 Å 31008 7e82 EMDB-31008, PDB-7e82: Cryo-EM structure of the flagellar rod with partial hook from Salmonella Method: EM (single particle) / Resolution: 3.3 Å
Chemicals	ChemComp-OCA: OCTANOIC ACID (CAPRYLIC ACID)
Source	salmonella typhimurium (strain lt2 / sgsc1412 / atcc 700720) (bacteria) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Keywords	MOTOR PROTEIN / Flagella / LP ring / FlgH / FlgI / Distal rod / FlgG / FlgF / Proximal rod / FlgC / FlgB / FliF / Export apparatus / FliE / MS ring / Hook / FlgE / Hook-basal body / Rod / Motor-hook