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TitleArchitecture of a SARS-CoV-2 mini replication and transcription complex.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 5874, Year 2020
Publish dateNov 18, 2020
AuthorsLiming Yan / Ying Zhang / Ji Ge / Litao Zheng / Yan Gao / Tao Wang / Zhihui Jia / Haofeng Wang / Yucen Huang / Mingyu Li / Quan Wang / Zihe Rao / Zhiyong Lou /
PubMed AbstractNon-structural proteins (nsp) constitute the SARS-CoV-2 replication and transcription complex (RTC) to play a pivotal role in the virus life cycle. Here we determine the atomic structure of a SARS- ...Non-structural proteins (nsp) constitute the SARS-CoV-2 replication and transcription complex (RTC) to play a pivotal role in the virus life cycle. Here we determine the atomic structure of a SARS-CoV-2 mini RTC, assembled by viral RNA-dependent RNA polymerase (RdRp, nsp12) with a template-primer RNA, nsp7 and nsp8, and two helicase molecules (nsp13-1 and nsp13-2), by cryo-electron microscopy. Two groups of mini RTCs with different conformations of nsp13-1 are identified. In both of them, nsp13-1 stabilizes overall architecture of the mini RTC by contacting with nsp13-2, which anchors the 5'-extension of RNA template, as well as interacting with nsp7-nsp8-nsp12-RNA. Orientation shifts of nsp13-1 results in its variable interactions with other components in two forms of mini RTC. The mutations on nsp13-1:nsp12 and nsp13-1:nsp13-2 interfaces prohibit the enhancement of helicase activity achieved by mini RTCs. These results provide an insight into how helicase couples with polymerase to facilitate its function in virus replication and transcription.
External linksNat Commun / PubMed:33208736 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.84 Å
Structure data

30492

7cxm

EMDB-30492, PDB-7cxm:
Architecture of a SARS-CoV-2 mini replication and transcription complex
Method: EM (single particle) / Resolution: 2.9 Å

30493

7cxn

EMDB-30493, PDB-7cxn:
Architecture of a SARS-CoV-2 mini replication and transcription complex
Method: EM (single particle) / Resolution: 3.84 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • severe acute respiratory syndrome coronavirus 2
KeywordsVIRAL PROTEIN/RNA / SARS-CoV-2 / RTC complex / VIRAL PROTEIN-RNA complex / RTC

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