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TitleStructural characterization of a neutralizing mAb H16.001, a potent candidate for a common potency assay for various HPV16 VLPs.
Journal, issue, pagesNPJ Vaccines, Vol. 5, Page 89, Year 2020
Publish dateSep 23, 2020
AuthorsWeijin Huang / Maozhou He / Tingting Ning / Jianhui Nie / Feng Zhang / Qingbing Zheng / Rui Zhang / Ying Xu / Ying Gu / Shaowei Li / Youchun Wang /
PubMed AbstractWith more human papillomavirus (HPV) virus-like particle (VLP) vaccines to hit the market in future, a monoclonal antibody (mAb) with preferably comparable reactivity against vaccines from different ...With more human papillomavirus (HPV) virus-like particle (VLP) vaccines to hit the market in future, a monoclonal antibody (mAb) with preferably comparable reactivity against vaccines from different expression systems and bioprocesses is urgently needed for the potency characterization. Among all mAbs against HPV16 collected, rabbit mAb H16.001 is potently neutralizing with the highest affinity, recognizes an immune-dominant epitope, and can comparably react with HPV16 vaccines from various sources. Cryo-electron microscopic (cryo-EM) structure demonstrated that 360 H16.001 Fabs could bind to HPV16 capsid in preferable binding manner without steric hindrance between neighboring Fabs, potentially supporting its identification for VLP structural integrity and utility in monitoring VLP structural probity. This structural analysis indicated that mAb H16.001 afforded unbiased potency characterization for various HPV16 vaccines and was potential for use in vaccine regulation practice. This study also showed a model process for selecting suitable mAbs for potency assays of other vaccines.
External linksNPJ Vaccines / PubMed:33042588 / PubMed Central
MethodsEM (single particle)
Resolution3.43 - 4.4 Å
Structure data

30414

7cn2

EMDB-30414, PDB-7cn2:
Subparticle refinement of human papillomavirus type 16 pesudovirus in complex with H16.001 Fab
Method: EM (single particle) / Resolution: 3.43 Å

EMDB-30415:
The cryo-EM structure of human papillomavirus type 16 pesudovirus in complex with Fab H16.001
Method: EM (single particle) / Resolution: 4.4 Å

Source
  • human papillomavirus type 16
  • oryctolagus cuniculus (rabbit)
KeywordsIMMUNE SYSTEM/VIRAL PROTEIN / Virus / immune complex / IMMUNE SYSTEM-VIRAL PROTEIN complex

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