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Structure paper

TitleStructure of a Minimal α-Carboxysome-Derived Shell and Its Utility in Enzyme Stabilization.
Journal, issue, pagesBiomacromolecules, Vol. 22, Issue 10, Page 4095-4109, Year 2021
Publish dateOct 11, 2021
AuthorsYong Quan Tan / Samson Ali / Bo Xue / Wei Zhe Teo / Lay Hiang Ling / Maybelle Kho Go / Hong Lv / Robert C Robinson / Akihiro Narita / Wen Shan Yew /
PubMed AbstractBacterial microcompartments are proteinaceous shells that encase specialized metabolic processes in bacteria. Recent advances in simplification of these intricate shells have encouraged ...Bacterial microcompartments are proteinaceous shells that encase specialized metabolic processes in bacteria. Recent advances in simplification of these intricate shells have encouraged bioengineering efforts. Here, we construct minimal shells derived from the α-carboxysome, which we term Cso-shell. Using cryogenic electron microscopy, the atomic-level structures of two shell forms were obtained, reinforcing notions of evolutionarily conserved features in bacterial microcompartment shell architecture. Encapsulation peptide sequences that facilitate loading of heterologous protein cargo within the shells were identified. We further provide a first demonstration in utilizing minimal bacterial microcompartment-derived shells for hosting heterologous enzymes. Cso-shells were found to stabilize enzymatic activities against heat shock, presence of methanol co-solvent, consecutive freeze-thawing, and alkaline environments. This study yields insights into α-carboxysome assembly and advances the utility of synthetic bacterial microcompartments as nanoreactors capable of stabilizing enzymes with varied properties and reaction chemistries.
External linksBiomacromolecules / PubMed:34384019
MethodsEM (single particle) / X-ray diffraction
Resolution2.7 - 3.24 Å
Structure data

30384

7ckb

EMDB-30384, PDB-7ckb:
Simplified Alpha-Carboxysome, T=3
Method: EM (single particle) / Resolution: 3.24 Å

30385

7ckc

EMDB-30385, PDB-7ckc:
Simplified Alpha-Carboxysome, T=4
Method: EM (single particle) / Resolution: 2.9 Å

PDB-7dhq:
Structure of Halothiobacillus neapolitanus Microcompartments Protein CsoS1D
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • halothiobacillus neapolitanus (bacteria)
  • halothiobacillus neapolitanus (strain atcc 23641 / c2) (bacteria)
KeywordsVIRUS LIKE PARTICLE / Bacterial Microcompartment / Alpha-carboxysome / STRUCTURAL PROTEIN / Microcompartments Protein / CsoS1D

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