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TitleA neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2.
Journal, issue, pagesScience, Vol. 369, Issue 6504, Page 650-655, Year 2020
Publish dateAug 7, 2020
AuthorsXiangyang Chi / Renhong Yan / Jun Zhang / Guanying Zhang / Yuanyuan Zhang / Meng Hao / Zhe Zhang / Pengfei Fan / Yunzhu Dong / Yilong Yang / Zhengshan Chen / Yingying Guo / Jinlong Zhang / Yaning Li / Xiaohong Song / Yi Chen / Lu Xia / Ling Fu / Lihua Hou / Junjie Xu / Changming Yu / Jianmin Li / Qiang Zhou / Wei Chen /
PubMed AbstractDeveloping therapeutics against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be guided by the distribution of epitopes, not only on the receptor binding domain (RBD) of the ...Developing therapeutics against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be guided by the distribution of epitopes, not only on the receptor binding domain (RBD) of the Spike (S) protein but also across the full Spike (S) protein. We isolated and characterized monoclonal antibodies (mAbs) from 10 convalescent COVID-19 patients. Three mAbs showed neutralizing activities against authentic SARS-CoV-2. One mAb, named 4A8, exhibits high neutralization potency against both authentic and pseudotyped SARS-CoV-2 but does not bind the RBD. We defined the epitope of 4A8 as the N-terminal domain (NTD) of the S protein by determining with cryo-eletron microscopy its structure in complex with the S protein to an overall resolution of 3.1 angstroms and local resolution of 3.3 angstroms for the 4A8-NTD interface. This points to the NTD as a promising target for therapeutic mAbs against COVID-19.
External linksScience / PubMed:32571838 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.3 Å
Structure data

30276

7c2l

EMDB-30276: cryo EM map of the S protein of SARS-CoV-2 in complex bound with 4A8
PDB-7c2l: S protein of SARS-CoV-2 in complex bound with 4A8
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-30277:
Cryo EM map of the interface between NTD of SARS-CoV-2 and 4A8
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
  • severe acute respiratory syndrome coronavirus 2
KeywordsMEMBRANE PROTEIN/IMMUNE SYSTEM / ACE2-B0AT1 complex / MEMBRANE PROTEIN / MEMBRANE PROTEIN-IMMUNE SYSTEM complex

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