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| Title | Ribosomes in RNA Granules Are Stalled on mRNA Sequences That Are Consensus Sites for FMRP Association. |
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| Journal, issue, pages | J Neurosci, Vol. 43, Issue 14, Page 2440-2459, Year 2023 |
| Publish date | Apr 5, 2023 |
 Authors | Mina N Anadolu / Jingyu Sun / Senthilkumar Kailasam / Kleanthi Chalkiadaki / Konstanze Krimbacher / Jewel T-Y Li / Teodora Markova / Seyed M Jafarnejad / Francois Lefebvre / Joaquin Ortega / Christos G Gkogkas / Wayne S Sossin /     |
| PubMed Abstract | Local translation in neurons is partly mediated by the reactivation of stalled polysomes. Stalled polysomes may be enriched within the granule fraction, defined as the pellet of sucrose gradients ...Local translation in neurons is partly mediated by the reactivation of stalled polysomes. Stalled polysomes may be enriched within the granule fraction, defined as the pellet of sucrose gradients used to separate polysomes from monosomes. The mechanism of how elongating ribosomes are reversibly stalled and unstalled on mRNAs is still unclear. In the present study, we characterize the ribosomes in the granule fraction using immunoblotting, cryogenic electron microscopy (cryo-EM), and ribosome profiling. We find that this fraction, isolated from 5-d-old rat brains of both sexes, is enriched in proteins implicated in stalled polysome function, such as the fragile X mental retardation protein (FMRP) and Up-frameshift mutation 1 homologue. Cryo-EM analysis of ribosomes in this fraction indicates they are stalled, mainly in the hybrid state. Ribosome profiling of this fraction reveals (1) an enrichment for footprint reads of mRNAs that interact with FMRPs and are associated with stalled polysomes, (2) an abundance of footprint reads derived from mRNAs of cytoskeletal proteins implicated in neuronal development, and (3) increased ribosome occupancy on mRNAs encoding RNA binding proteins. Compared with those usually found in ribosome profiling studies, the footprint reads were longer and were mapped to reproducible peaks in the mRNAs. These peaks were enriched in motifs previously associated with mRNAs cross-linked to FMRP , independently linking the ribosomes in the granule fraction to the ribosomes associated with FMRP in the cell. The data supports a model in which specific sequences in mRNAs act to stall ribosomes during translation elongation in neurons. Neurons send mRNAs to synapses in RNA granules, where they are not translated until an appropriate stimulus is given. Here, we characterize a granule fraction obtained from sucrose gradients and show that polysomes in this fraction are stalled on consensus sequences in a specific state of translational arrest with extended ribosome-protected fragments. This finding greatly increases our understanding of how neurons use specialized mechanisms to regulate translation and suggests that many studies on neuronal translation may need to be re-evaluated to include the large fraction of neuronal polysomes found in the pellet of sucrose gradients used to isolate polysomes. |
 External links | J Neurosci / PubMed:36849416 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 2.3 - 3.0 Å |
| Structure data |  EMDB-26517: Rat 80S ribosome purified from brain RNA granules. Class 2 40S subunit.  EMDB-26518: Rat 80S ribosome purified from brain RNA granules. Class 2 60S subunit.  EMDB-28726: Rat 80S ribosome purified from brain RNA granules. Class 1 40S subunit 2_5A resolution  EMDB-28727: Rat 80S ribosome purified from brain RNA granules. Class 1 60S subunit 2_5A resolution.  EMDB-29538: Rat 80S ribosome purified from brain RNA granules. Class 1 80S consensus  EMDB-29539: Rat 80S ribosome purified from brain RNA granules. Class 2 80S consensus |
| Source |
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Rattus norvegicus (Norway rat)