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TitleDynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates.
Journal, issue, pagesNat Struct Mol Biol, Vol. 32, Issue 3, Page 520-530, Year 2025
Publish dateJan 15, 2025
AuthorsMichael Uckelmann / Vita Levina / Cyntia Taveneau / Xiao Han Ng / Varun Pandey / Jasmine Martinez / Shweta Mendiratta / Justin Houx / Marion Boudes / Hari Venugopal / Sylvain Trépout / Alex J Fulcher / Qi Zhang / Sarena Flanigan / Minrui Li / Emma Sierecki / Yann Gambin / Partha Pratim Das / Oliver Bell / Alex de Marco / Chen Davidovich /
PubMed AbstractThe compaction of chromatin is a prevalent paradigm in gene repression. Chromatin compaction is commonly thought to repress transcription by restricting chromatin accessibility. However, the spatial ...The compaction of chromatin is a prevalent paradigm in gene repression. Chromatin compaction is commonly thought to repress transcription by restricting chromatin accessibility. However, the spatial organization and dynamics of chromatin compacted by gene-repressing factors are unknown. Here, using cryo-electron tomography, we solved the three-dimensional structure of chromatin condensed by the polycomb repressive complex 1 (PRC1) in a complex with CBX8. PRC1-condensed chromatin is porous and stabilized through multivalent dynamic interactions of PRC1 with chromatin. Mechanistically, positively charged residues on the internally disordered regions of CBX8 mask negative charges on the DNA to stabilize the condensed state of chromatin. Within condensates, PRC1 remains dynamic while maintaining a static chromatin structure. In differentiated mouse embryonic stem cells, CBX8-bound chromatin remains accessible. These findings challenge the idea of rigidly compacted polycomb domains and instead provide a mechanistic framework for dynamic and accessible PRC1-chromatin condensates.
External linksNat Struct Mol Biol / PubMed:39815045 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-29022: Reconstituted chromatin condensed by the PRC1-CBX8 complex
Method: EM (tomography)

EMDB-43554: In vitro reconstituted human chromatin
Method: EM (tomography)

Source
  • Homo sapiens (human)

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