EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Brominated lipid probes expose structural asymmetries in constricted membranes.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 30, Issue 2, Page 167-175, Year 2023
掲載日	2023年1月9日
著者	Frank R Moss / James Lincoff / Maxwell Tucker / Arshad Mohammed / Michael Grabe / Adam Frost /
PubMed 要旨	Lipids in biological membranes are thought to be functionally organized, but few experimental tools can probe nanoscale membrane structure. Using brominated lipids as contrast probes for cryo-EM and ...Lipids in biological membranes are thought to be functionally organized, but few experimental tools can probe nanoscale membrane structure. Using brominated lipids as contrast probes for cryo-EM and a model ESCRT-III membrane-remodeling system composed of human CHMP1B and IST1, we observed leaflet-level and protein-localized structural lipid patterns within highly constricted and thinned membrane nanotubes. These nanotubes differed markedly from protein-free, flat bilayers in leaflet thickness, lipid diffusion rates and lipid compositional and conformational asymmetries. Simulations and cryo-EM imaging of brominated stearoyl-docosahexanenoyl-phosphocholine showed how a pair of phenylalanine residues scored the outer leaflet with a helical hydrophobic defect where polyunsaturated docosahexaenoyl tails accumulated at the bilayer surface. Combining cryo-EM of halogenated lipids with molecular dynamics thus enables new characterizations of the composition and structure of membranes on molecular length scales.
リンク	Nat Struct Mol Biol / PubMed:36624348 / PubMed Central
手法	EM (らせん対称)
解像度	2.8 - 9.5 Å
構造データ	EMDB-27991: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 2.9 Å EMDB-28694: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 3.0 Å EMDB-28695: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated POPS probe 手法: EM (らせん対称) / 解像度: 2.8 Å EMDB-28696: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated POPS probe 手法: EM (らせん対称) / 解像度: 3.1 Å EMDB-28697: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated PIP2 probe 手法: EM (らせん対称) / 解像度: 3.2 Å EMDB-28698: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated PIP2 probe 手法: EM (らせん対称) / 解像度: 3.5 Å EMDB-28699: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated cholesterol probe 手法: EM (らせん対称) / 解像度: 3.8 Å EMDB-28700: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9L/F13L)+IST1 filament with no brominated lipids 手法: EM (らせん対称) / 解像度: 3.1 Å EMDB-28701: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with no brominated lipids 手法: EM (らせん対称) / 解像度: 3.7 Å EMDB-28702: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with iodinated cholesterol probe 手法: EM (らせん対称) / 解像度: 3.2 Å EMDB-28703: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with iodinated cholesterol probe 手法: EM (らせん対称) / 解像度: 3.1 Å EMDB-28704: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 25% brominated SDPC probe 手法: EM (らせん対称) / 解像度: 3.1 Å EMDB-28705: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with 25% brominated SDPC probe 手法: EM (らせん対称) / 解像度: 2.9 Å EMDB-28706: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 50% brominated SDPC probe 手法: EM (らせん対称) / 解像度: 2.9 Å EMDB-28707: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with 50% brominated SDPC probe 手法: EM (らせん対称) / 解像度: 3.0 Å EMDB-28708: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 0% cholesterol 手法: EM (らせん対称) / 解像度: 4.1 Å EMDB-28709: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 10% cholesterol 手法: EM (らせん対称) / 解像度: 4.0 Å EMDB-28710: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 20% cholesterol 手法: EM (らせん対称) / 解像度: 7.6 Å EMDB-28711: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 30% cholesterol 手法: EM (らせん対称) / 解像度: 4.4 Å EMDB-28712: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 40% cholesterol 手法: EM (らせん対称) / 解像度: 4.7 Å EMDB-28713: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9A/F13A)+IST1 filament with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 2.9 Å EMDB-28714: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9A/F133A)+IST1 filament with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 3.0 Å EMDB-28715: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9E/F13E)+IST1 filament with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 3.4 Å EMDB-28716: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9E/F13E)+IST1 filament with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 3.5 Å EMDB-28717: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9L/F13L)+IST1 filament with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 3.2 Å EMDB-28718: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9L/F13L)+IST1 filament with brominated SDPC probe 手法: EM (らせん対称) / 解像度: 2.9 Å EMDB-28719: CryoEM reconstruction of membrane-bound, right-handed, 34-subunit-per-turn CHMP1B+IST1 filament with POPC substituted for SDPC 手法: EM (らせん対称) / 解像度: 9.5 Å EMDB-28722: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated cholesterol probe 手法: EM (らせん対称) / 解像度: 4.3 Å
由来	Homo sapiens (ヒト)