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TitleStructures of β-adrenergic receptor in complex with Gs and ligands of different efficacies.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4095, Year 2022
Publish dateJul 14, 2022
AuthorsMinfei Su / Navid Paknejad / Lan Zhu / Jinan Wang / Hung Nguyen Do / Yinglong Miao / Wei Liu / Richard K Hite / Xin-Yun Huang /
PubMed AbstractG-protein-coupled receptors (GPCRs) receive signals from ligands with different efficacies, and transduce to heterotrimeric G-proteins to generate different degrees of physiological responses. ...G-protein-coupled receptors (GPCRs) receive signals from ligands with different efficacies, and transduce to heterotrimeric G-proteins to generate different degrees of physiological responses. Previous studies revealed how ligands with different efficacies activate GPCRs. Here, we investigate how a GPCR activates G-proteins upon binding ligands with different efficacies. We report the cryo-EM structures of β-adrenergic receptor (β-AR) in complex with Gs (GαGβGγ) and a partial agonist or a very weak partial agonist, and compare them to the β-AR-Gs structure in complex with a full agonist. Analyses reveal similar overall complex architecture, with local conformational differences. Cellular functional studies with mutations of β-AR residues show effects on the cellular signaling from β-AR to the cAMP response initiated by the three different ligands, with residue-specific functional differences. Biochemical investigations uncover that the intermediate state complex comprising β-AR and nucleotide-free Gs is more stable when binding a full agonist than a partial agonist. Molecular dynamics simulations support the local conformational flexibilities and different stabilities among the three complexes. These data provide insights into the ligand efficacy in the activation of GPCRs and G-proteins.
External linksNat Commun / PubMed:35835792 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 2.6 Å
Structure data

27328

8dcr

EMDB-27328, PDB-8dcr:
Cryo-EM structure of dobutamine-bound beta1-adrenergic receptor in complex with heterotrimeric Gs-protein
Method: EM (single particle) / Resolution: 2.6 Å

27329

8dcs

EMDB-27329, PDB-8dcs:
Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with heterotrimeric Gs-protein
Method: EM (single particle) / Resolution: 2.5 Å

Chemicals

ChemComp-Y00:
DOBUTAMINE / medication*YM / Dobutamine

ChemComp-P32:
4-{[(2S)-3-(tert-butylamino)-2-hydroxypropyl]oxy}-3H-indole-2-carbonitrile

Source
  • meleagris gallopavo (turkey)
  • bos taurus (cattle)
  • lama glama (llama)
KeywordsSIGNALING PROTEIN / beta1-adrenergic receptor / dobutamine / partial agonist / cyanopindolol

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