Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Benchmarking the ideal sample thickness in cryo-EM.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 118, Issue 49, Year 2021
Publish date	Dec 7, 2021
Authors	Michael W Martynowycz / Max T B Clabbers / Johan Unge / Johan Hattne / Tamir Gonen /
PubMed Abstract	The relationship between sample thickness and quality of data obtained is investigated by microcrystal electron diffraction (MicroED). Several electron microscopy (EM) grids containing proteinase K ...The relationship between sample thickness and quality of data obtained is investigated by microcrystal electron diffraction (MicroED). Several electron microscopy (EM) grids containing proteinase K microcrystals of similar sizes from the same crystallization batch were prepared. Each grid was transferred into a focused ion beam and a scanning electron microscope in which the crystals were then systematically thinned into lamellae between 95- and 1,650-nm thick. MicroED data were collected at either 120-, 200-, or 300-kV accelerating voltages. Lamellae thicknesses were expressed in multiples of the corresponding inelastic mean free path to allow the results from different acceleration voltages to be compared. The quality of the data and subsequently determined structures were assessed using standard crystallographic measures. Structures were reliably determined with similar quality from crystalline lamellae up to twice the inelastic mean free path. Lower resolution diffraction was observed at three times the mean free path for all three accelerating voltages, but the data quality was insufficient to yield structures. Finally, no coherent diffraction was observed from lamellae thicker than four times the calculated inelastic mean free path. This study benchmarks the ideal specimen thickness with implications for all cryo-EM methods.
External links	Proc Natl Acad Sci U S A / PubMed:34873060 / PubMed Central
Methods	EM (electron crystallography)
Resolution	1.85 - 2.9 Å
Structure data	25456 7svy EMDB-25456, PDB-7svy: MicroED structure of proteinase K from a 130 nm thick lamella measured at 120 kV Method: EM (electron crystallography) 25457 7svz EMDB-25457, PDB-7svz: MicroED structure of proteinase K from a 200 nm thick lamella measured at 120 kV Method: EM (electron crystallography) 25458 7sw0 EMDB-25458, PDB-7sw0: MicroED structure of proteinase K from a 325 nm thick lamella measured at 120 kV Method: EM (electron crystallography) 25459 7sw1 EMDB-25459, PDB-7sw1: MicroED structure of proteinase K from a 115 nm thick lamella measured at 200 kV Method: EM (electron crystallography) 25460 7sw2 EMDB-25460, PDB-7sw2: MicroED structure of proteinase K from a 130 nm thick lamella measured at 200 kV Method: EM (electron crystallography) 25461 7sw3 EMDB-25461, PDB-7sw3: MicroED structure of proteinase K from a 95 nm thick lamella measured at 200 kV Method: EM (electron crystallography) 25462 7sw4 EMDB-25462, PDB-7sw4: MicroED structure of proteinase K from a 540 nm thick lamella measured at 200 kV Method: EM (electron crystallography) 25463 7sw5 EMDB-25463, PDB-7sw5: MicroED structure of proteinase K from a 460 nm thick lamella measured at 200 kV Method: EM (electron crystallography) 25464 7sw6 EMDB-25464, PDB-7sw6: MicroED structure of proteinase K from a 260 nm thick lamella measured at 200 kV Method: EM (electron crystallography) 25465 7sw7 EMDB-25465, PDB-7sw7: MicroED structure of proteinase K from a 530 nm thick lamella measured at 200 kV Method: EM (electron crystallography) 25466 7sw8 EMDB-25466, PDB-7sw8: MicroED structure of proteinase K from a 150 nm thick lamella measured at 300 kV Method: EM (electron crystallography) 25467 7sw9 EMDB-25467, PDB-7sw9: MicroED structure of proteinase K from a 170 nm thick lamella measured at 300 kV Method: EM (electron crystallography) 25468 7swa EMDB-25468, PDB-7swa: MicroED structure of proteinase K from a 320 nm thick lamella measured at 300 kV Method: EM (electron crystallography) 25469 7swb EMDB-25469, PDB-7swb: MicroED structure of proteinase K from a 360 nm thick lamella measured at 300 kV Method: EM (electron crystallography) 25470 7swc EMDB-25470, PDB-7swc: MicroED structure of proteinase K from a 550 nm thick lamella measured at 300 kV Method: EM (electron crystallography)
Chemicals	ChemComp-HOH: WATER
Source	parengyodontium album (fungus)
Keywords	HYDROLASE / Serine protease