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TitleFabrication of membrane proteins in the form of native cell membrane nanoparticles using novel membrane active polymers.
Journal, issue, pagesNanoscale Adv, Vol. 5, Issue 21, Page 5932-5940, Year 2023
Publish dateOct 24, 2023
AuthorsThi Kim Hoang Trinh / Claudio Catalano / Youzhong Guo /
PubMed AbstractMembrane proteins are a widespread class of bio-macromolecules responsible for numerous vital biological processes and serve as therapeutic targets for a vast array of contemporary medications. For ...Membrane proteins are a widespread class of bio-macromolecules responsible for numerous vital biological processes and serve as therapeutic targets for a vast array of contemporary medications. For membrane protein isolation and purification, detergents have historically been used. Despite this, detergents frequently result in protein instability. Consequently, their application was limited. Recent detergent-free approaches have been invented. Among these, styrene-maleic acid lipid particle (SMALP), diisobutylene-maleic acid lipid particle (DIBMALP), and native cell membrane nanoparticle (NCMN) systems are the most prevalent. The NCMN system intends to create a library of membrane-active polymers suitable for high-resolution structure determination of membrane protein. Design, synthesis, characterization, and comparative application evaluations of three novel classes of NCMN polymers, NCMNP13-, NCMNP21-, and NCMNP21b-, are presented in this article. Although each NCMN polymer can solubilize distinct model membrane proteins and retain native lipids in NCMN particles, only the NCMNP21b- family produces lipid-protein particles with ideal buffer compatibility and high homogeneity suitable for single-particle cryo-EM analysis. NCMNP21b- polymers that generate high-quality NCMN particles are particularly desirable for membrane protein structural biology.
External linksNanoscale Adv / PubMed:37881706 / PubMed Central
MethodsEM (single particle)
Resolution3.69 Å
Structure data

EMDB-25400: Multidrug efflux pump subunit AcrB
Method: EM (single particle) / Resolution: 3.69 Å

Source
  • Escherichia coli (strain K12) (bacteria)
  • Escherichia coli K-12 (bacteria)

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