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-Structure paper
Title | An antibody class with a common CDRH3 motif broadly neutralizes sarbecoviruses. |
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Journal, issue, pages | Sci Transl Med, Vol. 14, Issue 646, Page eabn6859, Year 2022 |
Publish date | May 25, 2022 |
![]() | Lihong Liu / Sho Iketani / Yicheng Guo / Eswar R Reddem / Ryan G Casner / Manoj S Nair / Jian Yu / Jasper F-W Chan / Maple Wang / Gabriele Cerutti / Zhiteng Li / Nicholas C Morano / Candace D Castagna / Laura Corredor / Hin Chu / Shuofeng Yuan / Vincent Kwok-Man Poon / Chris Chun-Sing Chan / Zhiwei Chen / Yang Luo / Marcus Cunningham / Alejandro Chavez / Michael T Yin / David S Perlin / Moriya Tsuji / Kwok-Yung Yuen / Peter D Kwong / Zizhang Sheng / Yaoxing Huang / Lawrence Shapiro / David D Ho / ![]() ![]() |
PubMed Abstract | The devastation caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made clear the importance of pandemic preparedness. To address future zoonotic outbreaks due to related ...The devastation caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made clear the importance of pandemic preparedness. To address future zoonotic outbreaks due to related viruses in the sarbecovirus subgenus, we identified a human monoclonal antibody, 10-40, that neutralized or bound all sarbecoviruses tested in vitro and protected against SARS-CoV-2 and SARS-CoV in vivo. Comparative studies with other receptor-binding domain (RBD)-directed antibodies showed 10-40 to have the greatest breadth against sarbecoviruses, suggesting that 10-40 is a promising agent for pandemic preparedness. Moreover, structural analyses on 10-40 and similar antibodies not only defined an epitope cluster in the inner face of the RBD that is well conserved among sarbecoviruses but also uncovered a distinct antibody class with a common CDRH3 motif. Our analyses also suggested that elicitation of this class of antibodies may not be overly difficult, an observation that bodes well for the development of a pan-sarbecovirus vaccine. |
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Methods | EM (single particle) / X-ray diffraction |
Resolution | 1.53 - 6.5 Å |
Structure data | ![]() EMDB-25146: Broadly Neutralizing Antibody 10-40 in complex with prefusion SARS-CoV-2 B.1.351 Spike glycoprotein ![]() EMDB-25166: Antibody 10-28 in complex with prefusion SARS-CoV-2 B.1.351 Spike glycoprotein ![]() EMDB-25167: Antibody 11-11 in complex with prefusion SARS-CoV-2 B.1.351 Spike glycoprotein ![]() PDB-7sd5: ![]() PDB-7si2: ![]() PDB-7ttm: ![]() PDB-7ttx: ![]() PDB-7tty: |
Chemicals | ![]() ChemComp-HOH: ![]() ChemComp-NAG: |
Source |
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![]() | VIRAL PROTEIN/IMMUNE SYSTEM / COVID-19 / SARS-CoV-2 / Viral protein / Spike glycoprotein / Receptor Binding Protein / RBD / Neutralizing antibody / 10-40 / Fab / VIRAL PROTEIN-IMMUNE SYSTEM complex / 10-28 / SarbecoVirus / SCH014 RBD / potent / RaTG13 RBD / WIV1 RBD |