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TitleEntropy-regularized deconvolution of cellular cryotransmission electron tomograms.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 50, Year 2021
Publish dateDec 14, 2021
AuthorsMatthew Croxford / Michael Elbaum / Muthuvel Arigovindan / Zvi Kam / David Agard / Elizabeth Villa / John Sedat /
PubMed AbstractCryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in ...Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).
External linksProc Natl Acad Sci U S A / PubMed:34876518 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution25.0 - 30.0 Å
Structure data

EMDB-24416:
Microtubule subtomogram average, randomized azimuth and restricted angular search
Method: EM (subtomogram averaging) / Resolution: 25.0 Å

EMDB-24417:
Microtubule subtomogram average from particles with a uniform starting orientation with respect to the missing wedge
Method: EM (subtomogram averaging) / Resolution: 25.0 Å

EMDB-24418:
Microtubule subtomogram average of deconvolved particles, randomized starting azimuth and restricted angular search
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

EMDB-24419:
Microtubule subtomogram average of deconvolved rarticles, randomized starting azimuth relative to the missing wedge, and restricted angular search
Method: EM (subtomogram averaging) / Resolution: 25.0 Å

EMDB-24433:
Yeast Nucleus Tomogram
Method: EM (tomography)

EMDB-24434:
Deconvolved Yeast Nuclear Tomogram
Method: EM (tomography)

EMDB-24435:
Tomogram of HEK292 Inculsion Body
Method: EM (tomography)

EMDB-24436:
Deconvolved Tomogram of HEK293 Inclusion Body
Method: EM (tomography)

Source
  • Homo sapiens (human)
  • Saccharomyces cerevisiae (brewer's yeast)

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