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TitleThe architecture of yeast DNA polymerase ζ.
Journal, issue, pagesCell Rep, Vol. 5, Issue 1, Page 79-86, Year 2013
Publish dateOct 17, 2013
AuthorsYacob Gómez-Llorente / Radhika Malik / Rinku Jain / Jayati Roy Choudhury / Robert E Johnson / Louise Prakash / Satya Prakash / Iban Ubarretxena-Belandia / Aneel K Aggarwal /
PubMed AbstractDNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall ...DNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Polζ has recently been shown to form a stable four-subunit enzyme (Polζ-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Polδ. To understand the 3D architecture and assembly of Polζ and Polζ-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Polζ and Polζ-d are organized relative to each other. In particular, we show that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Polζ and Polζ-d and a structural framework for understanding their roles in DNA damage bypass.
External linksCell Rep / PubMed:24120860 / PubMed Central
MethodsEM (single particle)
Resolution23.0 Å
Structure data

EMDB-2409:
The architecture of yeast DNA polymerase 'zeta'. Electron microscopy reconstruction of the heterotetrameric complex Rev3-Rev7-Pol31-Pol32.
Method: EM (single particle) / Resolution: 23.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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