EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	High-resolution mapping of the neutralizing and binding specificities of polyclonal sera post-HIV Env trimer vaccination.
ジャーナル・号・ページ	Elife, Vol. 10, Year 2021
掲載日	2021年1月13日
著者	Adam S Dingens / Payal Pratap / Keara Malone / Sarah K Hilton / Thomas Ketas / Christopher A Cottrell / Julie Overbaugh / John P Moore / P J Klasse / Andrew B Ward / Jesse D Bloom /
PubMed 要旨	Mapping polyclonal serum responses is critical to rational vaccine design. However, most high-resolution mapping approaches involve isolating and characterizing individual antibodies, which ...Mapping polyclonal serum responses is critical to rational vaccine design. However, most high-resolution mapping approaches involve isolating and characterizing individual antibodies, which incompletely defines the polyclonal response. Here we use two complementary approaches to directly map the specificities of the neutralizing and binding antibodies of polyclonal anti-HIV-1 sera from rabbits immunized with BG505 Env SOSIP trimers. We used mutational antigenic profiling to determine how all mutations in Env affected viral neutralization and electron microscopy polyclonal epitope mapping (EMPEM) to directly visualize serum Fabs bound to Env trimers. The dominant neutralizing specificities were generally only a subset of the more diverse binding specificities. Additional differences between binding and neutralization reflected antigenicity differences between virus and soluble Env trimer. Furthermore, we refined residue-level epitope specificity directly from sera, revealing subtle differences across sera. Together, mutational antigenic profiling and EMPEM yield a holistic view of the binding and neutralizing specificity of polyclonal sera.
リンク	Elife / PubMed:33438580 / PubMed Central
手法	EM (単粒子)
解像度	20.0 Å
構造データ	EMDB-23366: Polyclonal Immune complex of Fab binding to BG505 SOSIP.664 from serum of rabbit 5724 at wk 26 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-23367: Polyclonal Immune complex of Fab binding to BG505 SOSIP.664 from serum of rabbit 5727 at wk 26 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-23368: Polyclonal Immune complex of Fab binding to BG505 SOSIP.v4.1 from serum of rabbit 2124 at wk 22 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-23369: Polyclonal Immune complex of Fab binding to BG505 SOSIP.v4.1 from serum of rabbit 2214 at wk 43 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-23370: Polyclonal Immune complex of Fab binding to BG505 SOSIP.v4.1 from serum of rabbit 2423 at wk 18 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-23371: Polyclonal Immune complex of Fab binding to BG505 SOSIP.v4.1 from serum of rabbit 2425 at wk 18 手法: EM (単粒子) / 解像度: 20.0 Å
由来	Oryctolagus cuniculus (ウサギ) Human immunodeficiency virus (ヒト免疫不全ウイルス)