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TitleEnhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike.
Journal, issue, pagesCell Rep, Vol. 35, Issue 1, Page 108933, Year 2021
Publish dateApr 6, 2021
AuthorsRonald Derking / Joel D Allen / Christopher A Cottrell / Kwinten Sliepen / Gemma E Seabright / Wen-Hsin Lee / Yoann Aldon / Kimmo Rantalainen / Aleksandar Antanasijevic / Jeffrey Copps / Anila Yasmeen / Albert Cupo / Victor M Cruz Portillo / Meliawati Poniman / Niki Bol / Patricia van der Woude / Steven W de Taeye / Tom L G M van den Kerkhof / P J Klasse / Gabriel Ozorowski / Marit J van Gils / John P Moore / Andrew B Ward / Max Crispin / Rogier W Sanders /
PubMed AbstractArtificial glycan holes on recombinant Env-based vaccines occur when a potential N-linked glycosylation site (PNGS) is under-occupied, but not on their viral counterparts. Native-like SOSIP trimers, ...Artificial glycan holes on recombinant Env-based vaccines occur when a potential N-linked glycosylation site (PNGS) is under-occupied, but not on their viral counterparts. Native-like SOSIP trimers, including clinical candidates, contain such holes in the glycan shield that induce strain-specific neutralizing antibodies (NAbs) or non-NAbs. To eliminate glycan holes and mimic the glycosylation of native BG505 Env, we replace all 12 NxS sequons on BG505 SOSIP with NxT. All PNGS, except N133 and N160, are nearly fully occupied. Occupancy of the N133 site is increased by changing N133 to NxS, whereas occupancy of the N160 site is restored by reverting the nearby N156 sequon to NxS. Hence, PNGS in close proximity, such as in the N133-N137 and N156-N160 pairs, affect each other's occupancy. We further apply this approach to improve the occupancy of several Env strains. Increasing glycan occupancy should reduce off-target immune responses to vaccine antigens.
External linksCell Rep / PubMed:33826885 / PubMed Central
MethodsEM (single particle)
Resolution4.5 - 7.1 Å
Structure data

EMDB-22170:
BG505 SOSIPv5.2 in complex with PGT122 and two RM20E1 Fabs
Method: EM (single particle) / Resolution: 5.2 Å

EMDB-22171:
BG505 SOSIPv5.2 in complex with PGT122 and three RM20E1 Fabs
Method: EM (single particle) / Resolution: 6.0 Å

EMDB-22172:
C3 symmetric BG505 SOSIPv5.2 in complex with PGT122 and three RM20E1 Fabs
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-22173:
BG505 SOSIPv5.2 in complex with PGT122 and one RM20E1 Fab
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-22178:
BG505 SOSIPv5.2 in complex with PGT122 and no RM20E1 Fabs
Method: EM (single particle) / Resolution: 7.1 Å

EMDB-22179:
C3 symmetric BG505 SOSIPv5.2 in complex with PGT122 and no RM20E1 Fabs
Method: EM (single particle) / Resolution: 6.0 Å

Source
  • Human immunodeficiency virus 1
  • Homo sapiens (human)
  • Macaca mulatta (Rhesus monkey)

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