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TitleCryo-EM reveals species-specific components within the Cag type IV secretion system core complex.
Journal, issue, pagesElife, Vol. 9, Year 2020
Publish dateSep 2, 2020
AuthorsMichael J Sheedlo / Jeong Min Chung / Neha Sawhney / Clarissa L Durie / Timothy L Cover / Melanie D Ohi / D Borden Lacy /
PubMed AbstractThe pathogenesis of -associated gastric cancer is dependent on delivery of CagA into host cells through a type IV secretion system (T4SS). The Cag T4SS includes a large membrane-spanning core ...The pathogenesis of -associated gastric cancer is dependent on delivery of CagA into host cells through a type IV secretion system (T4SS). The Cag T4SS includes a large membrane-spanning core complex containing five proteins, organized into an outer membrane cap (OMC), a periplasmic ring (PR) and a stalk. Here, we report cryo-EM reconstructions of a core complex lacking Cag3 and an improved map of the wild-type complex. We define the structures of two unique species-specific components (Cag3 and CagM) and show that Cag3 is structurally similar to CagT. Unexpectedly, components of the OMC are organized in a 1:1:2:2:5 molar ratio (CagY:CagX:CagT:CagM:Cag3). CagX and CagY are components of both the OMC and the PR and bridge the symmetry mismatch between these regions. These results reveal that assembly of the T4SS core complex is dependent on incorporation of interwoven species-specific components.
External linksElife / PubMed:32876048 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.5 Å
Structure data

22076

6x6k

EMDB-22076, PDB-6x6k:
Cryo-EM Structure of the Helicobacter pylori dCag3 OMC
Method: EM (single particle) / Resolution: 3.1 Å

22077

6x6l

EMDB-22077, PDB-6x6l:
Cryo-EM Structure of CagX and CagY within the dCag3 Helicobacter pylori PR
Method: EM (single particle) / Resolution: 3.0 Å

22081

6x6s

EMDB-22081, PDB-6x6s:
Cryo-EM Structure of the Helicobacter pylori OMC
Method: EM (single particle) / Resolution: 3.4 Å

PDB-6x6j:
Cryo-EM Structure of CagX and CagY within the Helicobacter pylori PR
Method: ELECTRON MICROSCOPY / Resolution: 3.5 Å

Source
  • helicobacter pylori (bacteria)
  • helicobacter pylori (strain atcc 700392 / 26695) (bacteria)
KeywordsPROTEIN TRANSPORT / Helicobacter / T4SS / Secretion / Cag

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