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TitleAcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 2730, Year 2020
Publish dateJun 1, 2020
AuthorsMarscha Hirschi / Wang-Ting Lu / Andrew Santiago-Frangos / Royce Wilkinson / Sarah M Golden / Alan R Davidson / Gabriel C Lander / Blake Wiedenheft /
PubMed AbstractBacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of ...Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems.
External linksNat Commun / PubMed:32483187 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 4.2 Å
Structure data

21516

6w1x

EMDB-21516, PDB-6w1x:
Cryo-EM structure of anti-CRISPR AcrIF9, bound to the type I-F crRNA-guided CRISPR surveillance complex
Method: EM (single particle) / Resolution: 3.9 Å

21517

6whi

EMDB-21517: Cryo-EM structure of anti-CRISPR AcrIF9 and dsDNA, bound to the type I-F crRNA-guided CRISPR surveillance complex
PDB-6whi: Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • pseudomonas aeruginosa (bacteria)
  • proteus penneri (bacteria)
KeywordsIMMUNE SYSTEM/RNA / type I-F CRISPR RNA-guided surveillance complex / Csy complex / IMMUNE SYSTEM-RNA complex / IMMUNE SYSTEM/DNA/RNA / IMMUNE SYSTEM-DNA-RNA complex

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