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TitleCryoEM map of Pseudomonas aeruginosa PilQ enables structural characterization of TsaP.
Journal, issue, pagesStructure, Vol. 29, Issue 5, Page 457-466.e4, Year 2021
Publish dateMay 6, 2021
AuthorsMatthew McCallum / Stephanie Tammam / John L Rubinstein / Lori L Burrows / P Lynne Howell /
PubMed AbstractThe type IV pilus machinery is a multi-protein complex that polymerizes and depolymerizes a pilus fiber used for attachment, twitching motility, phage adsorption, natural competence, protein ...The type IV pilus machinery is a multi-protein complex that polymerizes and depolymerizes a pilus fiber used for attachment, twitching motility, phage adsorption, natural competence, protein secretion, and surface-sensing. An outer membrane secretin pore is required for passage of the pilus fiber out of the cell. Herein, the structure of the tetradecameric secretin, PilQ, from the Pseudomonas aeruginosa type IVa pilus system was determined to 4.3 Å and 4.4 Å resolution in the presence and absence of C symmetric spikes, respectively. The heptameric spikes were found to be two tandem C-terminal domains of TsaP. TsaP forms a belt around PilQ and while it is not essential for twitching motility, overexpression of TsaP triggers a signal cascade upstream of PilY1 leading to cyclic di-GMP up-regulation. These results resolve the identity of the spikes identified with Proteobacterial PilQ homologs and may reveal a new component of the surface-sensing cyclic di-GMP signal cascade.
External linksStructure / PubMed:33338410
MethodsEM (single particle)
Resolution4.3 - 6.9 Å
Structure data

21152

6ve2

EMDB-21152, PDB-6ve2:
Tetradecameric PilQ bound by TsaP heptamer from Pseudomonas aeruginosa
Method: EM (single particle) / Resolution: 4.3 Å

21153

6ve3

EMDB-21153, PDB-6ve3:
Tetradecameric PilQ from Pseudomonas aeruginosa
Method: EM (single particle) / Resolution: 4.3 Å

21154

6ve4

EMDB-21154, PDB-6ve4:
Pentadecameric PilQ from Pseudomonas aeruginosa
Method: EM (single particle) / Resolution: 6.9 Å

Source
  • pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (bacteria)
KeywordsPROTEIN TRANSPORT / Type IV pilus / T4P / PilQ / TsaP / secretin / pilotin / type IVa pilus / T4aP / pilus / outer membrane / periplasm / bacterial secretion system

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