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TitleStructure of the thermo-sensitive TRP channel TRP1 from the alga Chlamydomonas reinhardtii.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 4180, Year 2019
Publish dateSep 13, 2019
AuthorsLuke L McGoldrick / Appu K Singh / Lusine Demirkhanyan / Ting-Yu Lin / Ryan G Casner / Eleonora Zakharian / Alexander I Sobolevsky /
PubMed AbstractAlgae produce the largest amount of oxygen on earth and are invaluable for human nutrition and biomedicine, as well as for the chemical industry, energy production and agriculture. The mechanisms by ...Algae produce the largest amount of oxygen on earth and are invaluable for human nutrition and biomedicine, as well as for the chemical industry, energy production and agriculture. The mechanisms by which algae can detect and respond to changes in their environments can rely on membrane receptors, including TRP ion channels. Here we present a 3.5-Å resolution cryo-EM structure of the transient receptor potential (TRP) channel crTRP1 from the alga Chlamydomonas reinhardtii that opens in response to increased temperature and is positively regulated by the membrane lipid PIP. The structure of crTRP1 significantly deviates from the structures of other TRP channels and has a unique 2-fold symmetrical rose-shape architecture with elbow domains and ankyrin repeat domains submerged and dipping into the membrane, respectively. Our study provides a structure of a TRP channel from a micro-organism and a structural framework for better understanding algae biology and TRP channel evolution.
External linksNat Commun / PubMed:31519888 / PubMed Central
MethodsEM (single particle)
Resolution3.45 - 3.53 Å
Structure data

20498

6pw4

EMDB-20498, PDB-6pw4:
Cryo-EM Structure of Thermo-Sensitive TRP Channel TRP1 from the Alga Chlamydomonas reinhardtii in Detergent
Method: EM (single particle) / Resolution: 3.53 Å

20499

6pw5

EMDB-20499, PDB-6pw5:
Cryo-EM Structure of Thermo-Sensitive TRP Channel TRP1 from the Alga Chlamydomonas reinhardtii in Nanodiscs
Method: EM (single particle) / Resolution: 3.45 Å

Chemicals

ChemComp-PIO:
[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate

ChemComp-PCW:
1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DOPC, phospholipid*YM

ChemComp-CPL:
1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

ChemComp-PIK:
(2S)-3-{[(R)-hydroxy{[(1R,2R,3S,4R,5R,6S)-2,3,6-trihydroxy-4,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dihexadecanoate

Source
  • chlamydomonas reinhardtii (plant)
KeywordsTRANSPORT PROTEIN / Ion Channels / Membrane Protein / TRP Channels

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