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TitleStructure and mechanism of monoclonal antibody binding to the junctional epitope of Plasmodium falciparum circumsporozoite protein.
Journal, issue, pagesPLoS Pathog, Vol. 16, Issue 3, Page e1008373, Year 2020
Publish dateMar 9, 2020
AuthorsDavid Oyen / Jonathan L Torres / Phillip C Aoto / Yevel Flores-Garcia / Špela Binter / Tossapol Pholcharee / Sean Carroll / Sini Reponen / Rachael Wash / Qi Liang / Franck Lemiale / Emily Locke / Allan Bradley / C Richter King / Daniel Emerling / Paul Kellam / Fidel Zavala / Andrew B Ward / Ian A Wilson /
PubMed AbstractLasting protection has long been a goal for malaria vaccines. The major surface antigen on Plasmodium falciparum sporozoites, the circumsporozoite protein (PfCSP), has been an attractive target for ...Lasting protection has long been a goal for malaria vaccines. The major surface antigen on Plasmodium falciparum sporozoites, the circumsporozoite protein (PfCSP), has been an attractive target for vaccine development and most protective antibodies studied to date interact with the central NANP repeat region of PfCSP. However, it remains unclear what structural and functional characteristics correlate with better protection by one antibody over another. Binding to the junctional region between the N-terminal domain and central NANP repeats has been proposed to result in superior protection: this region initiates with the only NPDP sequence followed immediately by NANP. Here, we isolated antibodies in Kymab mice immunized with full-length recombinant PfCSP and two protective antibodies were selected for further study with reactivity against the junctional region. X-ray and EM structures of two monoclonal antibodies, mAb667 and mAb668, shed light on their differential affinity and specificity for the junctional region. Importantly, these antibodies also bind to the NANP repeat region with equal or better affinity. A comparison with an NANP-only binding antibody (mAb317) revealed roughly similar but statistically distinct levels of protection against sporozoite challenge in mouse liver burden models, suggesting that junctional antibody protection might relate to the ability to also cross-react with the NANP repeat region. Our findings indicate that additional efforts are necessary to isolate a true junctional antibody with no or much reduced affinity to the NANP region to elucidate the role of the junctional epitope in protection.
External linksPLoS Pathog / PubMed:32150583 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.566 - 18.0 Å
Structure data

EMDB-20444:
Fab667 in complex with recombinant, shortened circumsporozoite protein
Method: EM (single particle) / Resolution: 18.0 Å

EMDB-20445:
Fab668 in complex with recombinant, shortened circumsporozoite protein
Method: EM (single particle) / Resolution: 17.0 Å

PDB-6pbv:
Crystal structure of Fab668 complex
Method: X-RAY DIFFRACTION / Resolution: 1.566 Å

PDB-6pbw:
Crystal structure of Fab667 complex
Method: X-RAY DIFFRACTION / Resolution: 2.058 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-GOL:
GLYCEROL

Source
  • homo sapiens (human)
  • plasmodium falciparum 3d7 (eukaryote)
KeywordsIMMUNE SYSTEM / Fab fragment

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