Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.
Journal, issue, pages	Structure, Vol. 27, Issue 10, Page 1594-11600.e2, Year 2019
Publish date	Oct 1, 2019
Authors	Michael W Martynowycz / Wei Zhao / Johan Hattne / Grant J Jensen / Tamir Gonen /
PubMed Abstract	Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction ...Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction limits the thickness of crystals that can be investigated by MicroED, mainly due to absorption. Recent studies have demonstrated that focused ion-beam (FIB) milling can thin crystals into ideal-sized lamellae; however, it is not clear how to best apply FIB milling for MicroED. Here, the effects of polishing the lamellae, whereby the last few nanometers are milled away using a low-current gallium beam, are explored in both the platinum-precoated and uncoated samples. Our results suggest that precoating samples with a thin layer of platinum followed by polishing the crystal surfaces prior to data collection consistently led to superior results as indicated by higher signal-to-noise ratio, higher resolution, and better refinement statistics. This study lays the foundation for routine and reproducible methodology for sample preparation in MicroED.
External links	Structure / PubMed:31422911 / PubMed Central
Methods	EM (electron crystallography)
Resolution	1.79 - 2.59 Å
Structure data	20356 6pkj EMDB-20356, PDB-6pkj: MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#2) Method: EM (electron crystallography) / Resolution: 2.17 Å 20357 6pkk EMDB-20357, PDB-6pkk: MicroED structure of proteinase K from an uncoated, single lamella at 2.18A resolution (#5) Method: EM (electron crystallography) / Resolution: 2.176 Å 20358 6pkl EMDB-20358, PDB-6pkl: MicroED structure of proteinase K from an uncoated, single lamella at 2.59A resolution (#7) Method: EM (electron crystallography) / Resolution: 2.59 Å 20359 6pkm EMDB-20359, PDB-6pkm: MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#8) Method: EM (electron crystallography) / Resolution: 2.17 Å 20360 6pkn EMDB-20360, PDB-6pkn: MicroED structure of proteinase K from an unpolished, platinum-coated, single lamella at 2.08A resolution (#9) Method: EM (electron crystallography) 20361 6pko EMDB-20361: MicroED structure of proteinase K from a platinum-coated, unpolished, single lamella at 2.07A resolution (#12) PDB-6pko: MicroED structure of proteinase K from a platinum coated, unpolished, single lamella at 2.07A resolution (#12) Method: EM (electron crystallography) / Resolution: 2.07 Å 20362 6pkp EMDB-20362, PDB-6pkp: MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.91A resolution (#10) Method: EM (electron crystallography) / Resolution: 1.91 Å 20363 6pkq EMDB-20363, PDB-6pkq: MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.85A resolution (#11) Method: EM (electron crystallography) / Resolution: 1.85 Å 20364 6pkr EMDB-20364, PDB-6pkr: MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.79A resolution (#13) Method: EM (electron crystallography) / Resolution: 1.79 Å 20365 6pks EMDB-20365, PDB-6pks: MicroED structure of proteinase K from low-dose merged lamellae that were not pre-coated with platinum 2.16A resolution (LD) Method: EM (electron crystallography) / Resolution: 2.16 Å 20366 6pkt EMDB-20366, PDB-6pkt: MicroED structure of proteinase K from merging low-dose, platinum pre-coated lamellae at 1.85A resolution (LDPT) Method: EM (electron crystallography) / Resolution: 1.85 Å
Chemicals	ChemComp-HOH: WATER
Source	parengyodontium album (fungus) Engyodontium album, Tritirachium album
Keywords	HYDROLASE