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TitleQualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.
Journal, issue, pagesStructure, Vol. 27, Issue 10, Page 1594-11600.e2, Year 2019
Publish dateOct 1, 2019
AuthorsMichael W Martynowycz / Wei Zhao / Johan Hattne / Grant J Jensen / Tamir Gonen /
PubMed AbstractMicrocrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction ...Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction limits the thickness of crystals that can be investigated by MicroED, mainly due to absorption. Recent studies have demonstrated that focused ion-beam (FIB) milling can thin crystals into ideal-sized lamellae; however, it is not clear how to best apply FIB milling for MicroED. Here, the effects of polishing the lamellae, whereby the last few nanometers are milled away using a low-current gallium beam, are explored in both the platinum-precoated and uncoated samples. Our results suggest that precoating samples with a thin layer of platinum followed by polishing the crystal surfaces prior to data collection consistently led to superior results as indicated by higher signal-to-noise ratio, higher resolution, and better refinement statistics. This study lays the foundation for routine and reproducible methodology for sample preparation in MicroED.
External linksStructure / PubMed:31422911 / PubMed Central
MethodsEM (electron crystallography)
Resolution1.79 - 2.59 Å
Structure data

EMDB-20356, PDB-6pkj:
MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#2)
Method: EM (electron crystallography) / Resolution: 2.17 Å

EMDB-20357, PDB-6pkk:
MicroED structure of proteinase K from an uncoated, single lamella at 2.18A resolution (#5)
Method: EM (electron crystallography) / Resolution: 2.176 Å

EMDB-20358, PDB-6pkl:
MicroED structure of proteinase K from an uncoated, single lamella at 2.59A resolution (#7)
Method: EM (electron crystallography) / Resolution: 2.59 Å

EMDB-20359, PDB-6pkm:
MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#8)
Method: EM (electron crystallography) / Resolution: 2.17 Å

EMDB-20360, PDB-6pkn:
MicroED structure of proteinase K from an unpolished, platinum-coated, single lamella at 2.08A resolution (#9)
Method: EM (electron crystallography)

EMDB-20361: MicroED structure of proteinase K from a platinum-coated, unpolished, single lamella at 2.07A resolution (#12)
PDB-6pko: MicroED structure of proteinase K from a platinum coated, unpolished, single lamella at 2.07A resolution (#12)
Method: EM (electron crystallography) / Resolution: 2.07 Å

EMDB-20362, PDB-6pkp:
MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.91A resolution (#10)
Method: EM (electron crystallography) / Resolution: 1.91 Å

EMDB-20363, PDB-6pkq:
MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.85A resolution (#11)
Method: EM (electron crystallography) / Resolution: 1.85 Å

EMDB-20364, PDB-6pkr:
MicroED structure of proteinase K from a platinum-coated, polished, single lamella at 1.79A resolution (#13)
Method: EM (electron crystallography) / Resolution: 1.79 Å

EMDB-20365, PDB-6pks:
MicroED structure of proteinase K from low-dose merged lamellae that were not pre-coated with platinum 2.16A resolution (LD)
Method: EM (electron crystallography) / Resolution: 2.16 Å

EMDB-20366, PDB-6pkt:
MicroED structure of proteinase K from merging low-dose, platinum pre-coated lamellae at 1.85A resolution (LDPT)
Method: EM (electron crystallography) / Resolution: 1.85 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • parengyodontium album (fungus)
  • Engyodontium album, Tritirachium album
KeywordsHYDROLASE

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