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| Title | Cryo electron tomography with volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen . |
|---|---|
| Journal, issue, pages | Elife, Vol. 8, Year 2019 |
| Publish date | Nov 11, 2019 |
 Authors | Simon Imhof / Jiayan Zhang / Hui Wang / Khanh Huy Bui / Hoangkim Nguyen / Ivo Atanasov / Wong H Hui / Shun Kai Yang / Z Hong Zhou / Kent L Hill /  |
| PubMed Abstract | The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are ...The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from , a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 Å for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in reflects adaptations to support unique motility needs in diverse host environments. |
 External links | Elife / PubMed:31710293 / PubMed Central |
| Methods | EM (subtomogram averaging) |
| Resolution | 21.0 - 35.6 Å |
| Structure data |  EMDB-20012:  EMDB-20013:  EMDB-20014: |
| Source |
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Trypanosoma brucei brucei (eukaryote)