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TitleElectron transfer in the respiratory chain at low salinity.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 8241, Year 2024
Publish dateSep 19, 2024
AuthorsAna Paula Lobez / Fei Wu / Justin M Di Trani / John L Rubinstein / Mikael Oliveberg / Peter Brzezinski / Agnes Moe /
PubMed AbstractRecent studies have established that cellular electrostatic interactions are more influential than assumed previously. Here, we use cryo-EM and perform steady-state kinetic studies to investigate ...Recent studies have established that cellular electrostatic interactions are more influential than assumed previously. Here, we use cryo-EM and perform steady-state kinetic studies to investigate electrostatic interactions between cytochrome (cyt.) c and the complex (C) III-IV supercomplex from Saccharomyces cerevisiae at low salinity. The kinetic studies show a sharp transition with a Hill coefficient ≥2, which together with the cryo-EM data at 2.4 Å resolution indicate multiple cyt. c molecules bound along the supercomplex surface. Negatively charged loops of CIII subunits Qcr6 and Qcr9 become structured to interact with cyt. c. In addition, the higher resolution allows us to identify water molecules in proton pathways of CIV and, to the best of our knowledge, previously unresolved cardiolipin molecules. In conclusion, the lowered electrostatic screening renders engagement of multiple cyt. c molecules that are directed by electrostatically structured CIII loops to conduct electron transfer between CIII and CIV.
External linksNat Commun / PubMed:39300056 / PubMed Central
MethodsEM (single particle)
Resolution2.3 - 2.5 Å
Structure data

EMDB-19963, PDB-9etz:
III2IV respiratory supercomplex from Saccharomyces cerevisiae
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-19964: III2IV respiratory supercomplex from Saccharomyces cerevisiae (consensus map)
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-19965: Local refinement of CIII2 from Saccharomyces cerevisiae
Method: EM (single particle) / Resolution: 2.3 Å

EMDB-19966: Local refinement of CIV from Saccharomyces cerevisiae
Method: EM (single particle) / Resolution: 2.5 Å

Chemicals

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-PEF:
DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

ChemComp-PCF:
1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE

ChemComp-UQ6:
5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL

ChemComp-HEC:
HEME C

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-CU:
COPPER (II) ION

ChemComp-HEA:
HEME-A

ChemComp-CA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-CUA:
DINUCLEAR COPPER ION

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsMEMBRANE PROTEIN / Respiration / supercomplex

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