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-Structure paper
Title | 3D structure of the Yersinia entomophaga toxin complex and implications for insecticidal activity. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 108, Issue 51, Page 20544-20549, Year 2011 |
Publish date | Dec 20, 2011 |
Authors | Michael J Landsberg / Sandra A Jones / Rosalba Rothnagel / Jason N Busby / Sean D G Marshall / Robert M Simpson / J Shaun Lott / Ben Hankamer / Mark R H Hurst / |
PubMed Abstract | Toxin complex (Tc) proteins are a class of bacterial protein toxins that form large, multisubunit complexes. Comprising TcA, B, and C components, they are of great interest because many exhibit ...Toxin complex (Tc) proteins are a class of bacterial protein toxins that form large, multisubunit complexes. Comprising TcA, B, and C components, they are of great interest because many exhibit potent insecticidal activity. Here we report the structure of a novel Tc, Yen-Tc, isolated from the bacterium Yersinia entomophaga MH96, which differs from the majority of bacterially derived Tcs in that it exhibits oral activity toward a broad range of insect pests, including the diamondback moth (Plutella xylostella). We have determined the structure of the Yen-Tc using single particle electron microscopy and studied its mechanism of toxicity by comparative analyses of two variants of the complex exhibiting different toxicity profiles. We show that the A subunits form the basis of a fivefold symmetric assembly that differs substantially in structure and subunit arrangement from its most well characterized homologue, the Xenorhabdus nematophila toxin XptA1. Histopathological and quantitative dose response analyses identify the B and C subunits, which map to a single, surface-accessible region of the structure, as the sole determinants of toxicity. Finally, we show that the assembled Yen-Tc has endochitinase activity and attribute this to putative chitinase subunits that decorate the surface of the TcA scaffold, an observation that may explain the oral toxicity associated with the complex. |
External links | Proc Natl Acad Sci U S A / PubMed:22158901 / PubMed Central |
Methods | EM (single particle) |
Resolution | 17.0 Å |
Structure data | EMDB-1978: |
Source |
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