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TitleCryo-EM structure of the R388 plasmid conjugative pilus reveals a helical polymer characterized by an unusual pilin/phospholipid binary complex.
Journal, issue, pagesStructure, Vol. 32, Issue 9, Page 1335-11347.e5, Year 2024
Publish dateSep 5, 2024
AuthorsAbhinav K Vadakkepat / Songlin Xue / Adam Redzej / Terry K Smith / Brian T Ho / Gabriel Waksman /
PubMed AbstractBacterial conjugation is a process by which DNA is transferred unidirectionally from a donor cell to a recipient cell. It is the main means by which antibiotic resistance genes spread among bacterial ...Bacterial conjugation is a process by which DNA is transferred unidirectionally from a donor cell to a recipient cell. It is the main means by which antibiotic resistance genes spread among bacterial populations. It is crucially dependent upon the elaboration of an extracellular appendage, termed "pilus," by a large double-membrane-spanning secretion system termed conjugative "type IV secretion system." Here we present the structure of the conjugative pilus encoded by the R388 plasmid. We demonstrate that, as opposed to all conjugative pili produced so far for cryoelectron microscopy (cryo-EM) structure determination, the conjugative pilus encoded by the R388 plasmid is greatly stimulated by the presence of recipient cells. Comparison of its cryo-EM structure with existing conjugative pilus structures highlights a number of important differences between the R388 pilus structure and that of its homologs, the most prominent being the highly distinctive conformation of its bound lipid.
External linksStructure / PubMed:39002540
MethodsEM (helical sym.)
Resolution3.39 Å
Structure data

19758

8s6h

EMDB-19758, PDB-8s6h:
Cryo-EM Structure of the R388 plasmid conjugative pilus reveals a helical polymer characterised by an unusual pilin/phospholipid binary complex
Method: EM (helical sym.) / Resolution: 3.39 Å

Chemicals

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

Source
  • Escherichia coli (E. coli)
  • escherichia coli hb101 (bacteria)
KeywordsPROTEIN FIBRIL / Conjugal transfer protein / VirB2 / Conjugative pilus / Type-4 secretion system fibre / Conduit for horizontal gene transfer / anti-microbial resistance

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