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TitleVimentin filaments integrate low-complexity domains in a complex helical structure.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 6, Page 939-949, Year 2024
Publish dateApr 17, 2024
AuthorsMatthias Eibauer / Miriam S Weber / Rafael Kronenberg-Tenga / Charlie T Beales / Rajaa Boujemaa-Paterski / Yagmur Turgay / Suganya Sivagurunathan / Julia Kraxner / Sarah Köster / Robert D Goldman / Ohad Medalia /
PubMed AbstractIntermediate filaments (IFs) are integral components of the cytoskeleton. They provide cells with tissue-specific mechanical properties and are involved in numerous cellular processes. Due to their ...Intermediate filaments (IFs) are integral components of the cytoskeleton. They provide cells with tissue-specific mechanical properties and are involved in numerous cellular processes. Due to their intricate architecture, a 3D structure of IFs has remained elusive. Here we use cryo-focused ion-beam milling, cryo-electron microscopy and tomography to obtain a 3D structure of vimentin IFs (VIFs). VIFs assemble into a modular, intertwined and flexible helical structure of 40 α-helices in cross-section, organized into five protofibrils. Surprisingly, the intrinsically disordered head domains form a fiber in the lumen of VIFs, while the intrinsically disordered tails form lateral connections between the protofibrils. Our findings demonstrate how protein domains of low sequence complexity can complement well-folded protein domains to construct a biopolymer with striking mechanical strength and stretchability.
External linksNat Struct Mol Biol / PubMed:38632361 / PubMed Central
MethodsEM (helical sym.) / EM (subtomogram averaging)
Resolution7.2 - 20.2 Å
Structure data

16844

8rve

EMDB-16844: Vimentin intermediate filament structure
PDB-8rve: Vimentin intermediate filament
Method: EM (helical sym.) / Resolution: 7.2 Å

EMDB-19562: Vimentin intermediate filament protofibril stoichiometry
Method: EM (subtomogram averaging) / Resolution: 20.2 Å

EMDB-19563: Vimentin intermediate filament structure (delta tail)
Method: EM (helical sym.) / Resolution: 14.0 Å

Source
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / vimentin / intermediate filament / cytoskeleton

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