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-Structure paper
Title | Cryo-EM structure of a conjugative type 4 secretion system identifies a molecular switch regulating conjugative pilus biogenesis |
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Journal, issue, pages | To Be Published, Year 2024 |
Publish date | Jan 25, 2024 (structure data deposition date) |
Authors | Mace K / Waksman G |
External links | To Be Published / Search PubMed |
Methods | EM (single particle) |
Resolution | 2.46 - 6.22 Å |
Structure data | EMDB-19478: O-Layer C14 at 2.46A - Local refinement with C14 symmetry of the O-layer of the outer membrane core complex from the fully-assembled R388 type IV secretion system. EMDB-19479: I-Layer C16 at 2.69A - Local refinement with C16 symmetry of the I-layer of the outer membrane core complex from the fully-assembled R388 type IV secretion system. EMDB-19480: Conformation-A OMCC at 3.18A - Refinement without symmetry of the outer membrane core complex from the fully-assembled R388 type IV secretion system. EMDB-19481, PDB-8rt7: EMDB-19482: Conformation-C OMCC at 3.05A - Refinement without symmetry of the outer membrane core complex from the fully-assembled R388 type IV secretion system. EMDB-19483: Stalk C5 at 2.97A - Local refinement with C5 symmetry of the Stalk complex from the fully-assembled R388 type IV secretion system. EMDB-19484: Arches-protomer at 6.22A - Refinement without symmetry of the Arches-protomer complex from the fully-assembled R388 type IV secretion system. EMDB-19485: Extended inner membrne complex (IMC) protomer at 3.83A - Refinement without symmetry of the extended IMC-protomer complex from the fully-assembled R388 type IV secretion system. EMDB-19488: Stalk-Arches-IMC at 4.33A - Refinement without symmetry of the Stalk-Arches-IMC from the fully-assembled R388 type IV secretion. |
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Keywords | MEMBRANE PROTEIN / type IV secretion system type 4 secretion system T4SS O-layer core complex outer membrane complex R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB10 VirB9 VirB7 TrwE TrwF TrwH / type IV secretion system type 4 secretion system T4SS I-layer core complex outer membrane complex R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB10 VirB9 TrwE TrwF / type IV secretion system type 4 secretion system T4SS OMCC Conformation-A core complex outer membrane complex R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB10 VirB9 VirB7 TrwE TrwF TrwH / type IV secretion system type 4 secretion system T4SS OMCC Conformation-B core complex outer membrane complex R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB10 VirB9 VirB7 TrwE TrwF TrwH / type IV secretion system type 4 secretion system T4SS OMCC Conformation-C core complex outer membrane complex R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB10 VirB9 VirB7 TrwE TrwF TrwH / type IV secretion system type 4 secretion system T4SS Stalk R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB5 VirB6 TrwI TrwJ / type IV secretion system type 4 secretion system T4SS Arches Arches-protomer R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB8 VirB10 TrwG TrwE / type IV secretion system type 4 secretion system T4SS inner membrane complex IMC IMC-protomer Extended IMC R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB3 VirB4 VirB6 VirB8 VirB10 TrwM TrwK TrwI TrwG TrwE / type IV secretion system type 4 secretion system T4SS Arches Stalk inner membrane complex IMC R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB3 VirB4 VirB5 VirB6 VirB8 VirB10 TrwM TrwK TrwJ TrwI TrwG TrwE |