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TitleThe structure of inactivated mature tick-borne encephalitis virus at 3.0 Å resolution.
Journal, issue, pagesEmerg Microbes Infect, Vol. 13, Issue 1, Page 2313849, Year 2024
Publish dateMar 11, 2024
AuthorsEvgeny B Pichkur / Mikhail F Vorovitch / Alla L Ivanova / Elena V Protopopova / Valery B Loktev / Dmitry I Osolodkin / Aydar A Ishmukhametov / Valeriya R Samygina /
PubMed AbstractTick-borne encephalitis virus (TBEV) causes a severe disease, tick-borne encephalitis (TBE), that has a substantial epidemiological importance for Northern Eurasia. Between 10,000 and 15,000 TBE ...Tick-borne encephalitis virus (TBEV) causes a severe disease, tick-borne encephalitis (TBE), that has a substantial epidemiological importance for Northern Eurasia. Between 10,000 and 15,000 TBE cases are registered annually despite the availability of effective formaldehyde-inactivated full-virion vaccines due to insufficient vaccination coverage, as well as sporadic cases of vaccine breakthrough. The development of improved vaccines would benefit from the atomic resolution structure of the antigen. Here we report the refined single-particle cryo-electron microscopy (cryo-EM) structure of the inactivated mature TBEV vaccine strain Sofjin-Chumakov (Far-Eastern subtype) at a resolution of 3.0 Å. The increase of the resolution with respect to the previously published structures of TBEV strains Hypr and Kuutsalo-14 (European subtype) was reached due to improvement of the virus sample quality achieved by the optimized preparation methods. All the surface epitopes of TBEV were structurally conserved in the inactivated virions. ELISA studies with monoclonal antibodies supported the hypothesis of TBEV protein shell cross-linking upon inactivation with formaldehyde.
External linksEmerg Microbes Infect / PubMed:38465849 / PubMed Central
MethodsEM (single particle)
Resolution3.03 - 3.6 Å
Structure data

18614

8qrh

EMDB-18614, PDB-8qrh:
Inactivated tick-borne encephalitis virus (TBEV) vaccine strain Sofjin-Chumakov
Method: EM (single particle) / Resolution: 3.6 Å

19003

8r8l

EMDB-19003, PDB-8r8l:
The structure of inactivated mature tick-borne encephalitis virus
Method: EM (single particle) / Resolution: 3.03 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • orthoflavivirus encephalitidis
  • tick-borne encephalitis virus (strain sofjin)
KeywordsVIRAL PROTEIN / TBEV / flavivirus / virion envelope / VIRUS / cryo-EM / Tick-Borne Encephalitis Virus

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