Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM observation of the amyloid key structure of polymorphic TDP-43 amyloid fibrils.
Journal, issue, pages	Nat Commun, Vol. 15, Issue 1, Page 486, Year 2024
Publish date	Jan 12, 2024
Authors	Kartikay Sharma / Fabian Stockert / Jayakrishna Shenoy / Mélanie Berbon / Muhammed Bilal Abdul-Shukkoor / Birgit Habenstein / Antoine Loquet / Matthias Schmidt / Marcus Fändrich /
PubMed Abstract	The transactive response DNA-binding protein-43 (TDP-43) is a multi-facet protein involved in phase separation, RNA-binding, and alternative splicing. In the context of neurodegenerative diseases, ...The transactive response DNA-binding protein-43 (TDP-43) is a multi-facet protein involved in phase separation, RNA-binding, and alternative splicing. In the context of neurodegenerative diseases, abnormal aggregation of TDP-43 has been linked to amyotrophic lateral sclerosis and frontotemporal lobar degeneration through the aggregation of its C-terminal domain. Here, we report a cryo-electron microscopy (cryo-EM)-based structural characterization of TDP-43 fibrils obtained from the full-length protein. We find that the fibrils are polymorphic and contain three different amyloid structures. The structures differ in the number and relative orientation of the protofilaments, although they share a similar fold containing an amyloid key motif. The observed fibril structures differ from previously described conformations of TDP-43 fibrils and help to better understand the structural landscape of the amyloid fibril structures derived from this protein.
External links	Nat Commun / PubMed:38212334 / PubMed Central
Methods	EM (helical sym.)
Resolution	3.76 - 4.05 Å
Structure data	18715 8qx9 EMDB-18715, PDB-8qx9: TDP-43 amyloid fibrils: Morphology-1a Method: EM (helical sym.) / Resolution: 3.76 Å 18716 8qxa EMDB-18716, PDB-8qxa: TDP-43 amyloid fibrils: Morphology-1b Method: EM (helical sym.) / Resolution: 4.05 Å 18717 8qxb EMDB-18717, PDB-8qxb: TDP-43 amyloid fibrils: Morphology-2 Method: EM (helical sym.) / Resolution: 3.86 Å
Source	homo sapiens (human)
Keywords	PROTEIN FIBRIL / Amyloidosis / Protein misfolding disease / Amyloid fibrils / Cryo electron microscopy / Amyloid key